IL-3 ELISA Kit
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- Target Alle IL-3 ELISA Kits anzeigen
- IL-3 (Interleukin 3 (IL-3))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Applikation
- ELISA
- Verwendungszweck
- Human Interleukin 3 (IL-3) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 3 (IL-3) concentrations in cell culture supernates, serum, and plasma.
- Proben
- Serum, Plasma, Cell Culture Cells
- Analytische Methode
- Quantitative
- Sensitivität
- 15 pg/ml
- Produktmerkmale
- Human Interleukin 3 (IL-3) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Probenmenge
- 100 μL
- Testdauer
- 3.5 h
- Plattentyp
- Pre-coated
- Protokoll
- Human Interleukin 3 (IL-3) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-3 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-3 is added to the wells and binds to the combination of capture antibody- IL-3 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-3 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-3 standard dilutions and IL-3 sample concentration determined.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- 4 °C
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- Target Alle IL-3 ELISA Kits anzeigen
- IL-3 (Interleukin 3 (IL-3))
- Andere Bezeichnung
- IL-3 (IL-3 Produkte)
- Synonyme
- BPA ELISA Kit, Csfmu ELISA Kit, HCGF ELISA Kit, Il-3 ELISA Kit, MCGF ELISA Kit, PSF ELISA Kit, IL-3 ELISA Kit, MULTI-CSF ELISA Kit, interleukin 3 ELISA Kit, interleukin 3 (colony-stimulating factor, multiple) ELISA Kit, Il3 ELISA Kit, IL3 ELISA Kit
- Pathways
- JAK-STAT Signalweg, Regulation of Carbohydrate Metabolic Process, Autophagie
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