MGMT ELISA Kit

Produktnummer ABIN2044129

Produktdetails MGMT ELISA Kit

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12-5000 pg/mL

Untere Nachweisgrenze: 78.12 pg/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 27 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: O-6-Methylguanine DNA Methyltransferase (MGMT) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to O-6-Methylguanine DNA Methyltransferase (MGMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to O-6-Methylguanine DNA Methyltransferase (MGMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain O-6-Methylguanine DNA Methyltransferase (MGMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of O-6-Methylguanine DNA Methyltransferase (MGMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Andere Bezeichnung: O-6-Methylguanine DNA Methyltransferase (MGMT) (MGMT Produkte)

Synonyme: AGT ELISA Kit, AI267024 ELISA Kit, Agat ELISA Kit, O-6-methylguanine-DNA methyltransferase ELISA Kit, MGMT ELISA Kit, Mgmt ELISA Kit

Pathways: DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus