anti-Human Serotonin Receptor 6 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended Serotonin Receptor 6 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Serotonin Receptor 6 (HTR6) Antikörper
  • 5-HT6
  • 5-HT6R
  • 5-hydroxytryptamine (serotonin) receptor 6
  • 5-hydroxytryptamine receptor 6
  • Htr6
  • HTR6
Epitop
C-Term
8
3
2
1
1
1
1
1
1
Reaktivität
Hund, Hamster, Pferd, Human, Affe, Ratte (Rattus)
22
10
10
1
1
1
1
1
Wirt
Kaninchen
24
Klonalität
Polyklonal
Applikation
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
15
9
6
5
2
1
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN1048968
$ 484.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
7 ABIN1048967 IHC IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0
4 ABIN614849 IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0
4 ABIN1735625 IHC (p) Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN2765675 IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN2765674 IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0

General

Antigen Serotonin Receptor 6 (HTR6) Antikörper
Epitop C-Term
(8), (3), (2), (1), (1), (1), (1), (1), (1)
Reaktivität Hund, Hamster, Pferd, Human, Affe, Ratte (Rattus)
(22), (10), (10), (1), (1), (1), (1), (1)
Wirt Kaninchen
(24)
Klonalität
Polyklonal
Applikation Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(15), (9), (6), (5), (2), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Serotonin Receptor 6 Antikörper

Target Details Serotonin Receptor 6 Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human HTR6 / 5-HT36. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Rat, Hamster, Panda, Dog, Horse (100%) Mouse, Elephant, Bovine, Bat, Rabbit (94%) Stickleback, Pufferfish (88%) Turkey, Chicken, Xenopus, Zebrafish (82%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 17 amino acid peptide from C-terminus of human HTR6 / 5-HT36. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Rat, Hamster, Panda, Dog, Horse (100%), Mouse, Elephant, Bovine, Bat, Rabbit (94%), Stickleback, Pufferfish (88%), Turkey, Chicken, Xenopus, Zebrafish (82%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details Serotonin Receptor 6

Produktdetails anti-Serotonin Receptor 6 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung HTR6 / 5-HT6 Receptor (HTR6 Antibody Abstract)
Hintergrund Name/Gene ID: HTR6
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR6, 5-HT6, 5-HT6R, 5-HT-6, 5-hydroxytryptamine receptor 6, 5-HT6 receptor, 5-HT6 serotonin receptor, 5-hydroxytryptamine 6 receptor, Serotonin 6 receptor, Serotonin receptor 6, Serotonin 5-HT-6 receptor, Serotonin type 6 receptor
Gen-ID 3362
Pathways JAK-STAT Signalweg

Anwendungsinformationen

Produktdetails anti-Serotonin Receptor 6 Antikörper Target Details Serotonin Receptor 6 Handhabung Bilder zurück nach oben
Applikations-hinweise Approved: IHC, IHC-P (10 - 20 μg/mL)
Kommentare

Target Species of Antibody: Human

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Serotonin Receptor 6 Antikörper Target Details Serotonin Receptor 6 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-Serotonin Receptor 6 Antikörper Target Details Serotonin Receptor 6 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Serotonin Receptor 6 (HTR6) (C-Term) antibody (ABIN1048968) Human, Brain, Cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE)
Immunohistochemistry (IHC) image for anti-Serotonin Receptor 6 (HTR6) (C-Term) antibody (ABIN1048968) Anti-HTR6 / 5-HT6 antibody ABIN1048968 IHC staining of human brain, neuron. Immunohi...
Immunohistochemistry (IHC) image for anti-Serotonin Receptor 6 (HTR6) (C-Term) antibody (ABIN1048968) Human, Brain, Cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE)