anti-Human Serotonin Receptor 6 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended Serotonin Receptor 6 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Serotonin Receptor 6 (HTR6) Antikörper
  • 5-HT6
  • 5-HT6R
  • 5-hydroxytryptamine (serotonin) receptor 6
  • 5-hydroxytryptamine receptor 6
  • Htr6
  • HTR6
Epitop
C-Term
8
3
2
1
1
1
1
1
1
Reaktivität
Human
22
11
10
2
2
2
2
1
Wirt
Kaninchen
24
Klonalität
Polyklonal
Applikation
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
15
9
6
5
2
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN1048967
$ 484.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN1048968 IHC IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN5563572 IHC (p) Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN614849 IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN1735625 IHC (p) Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN2765674 IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN2765675 IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0

General

Antigen Serotonin Receptor 6 (HTR6) Antikörper
Epitop C-Term
(8), (3), (2), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(22), (11), (10), (2), (2), (2), (2), (1)
Wirt Kaninchen
(24)
Klonalität
Polyklonal
Applikation Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(15), (9), (6), (5), (2), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Serotonin Receptor 6 Antikörper

Target Details Serotonin Receptor 6 Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human HTR6 / 5-HT6. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla (100%) Monkey, Horse (94%) Gibbon, Dog, Rabbit (88%) Elephant (82%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 17 amino acid peptide from C-terminus of human HTR6 / 5-HT6. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Monkey, Horse (94%), Gibbon, Dog, Rabbit (88%), Elephant (82%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details Serotonin Receptor 6

Produktdetails anti-Serotonin Receptor 6 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung HTR6 / 5-HT6 Receptor (HTR6 Antibody Abstract)
Hintergrund Name/Gene ID: HTR6
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR6, 5-HT6, 5-HT6R, 5-HT-6, 5-hydroxytryptamine receptor 6, 5-HT6 receptor, 5-HT6 serotonin receptor, 5-hydroxytryptamine 6 receptor, Serotonin 6 receptor, Serotonin receptor 6, Serotonin 5-HT-6 receptor, Serotonin type 6 receptor
Gen-ID 3362
UniProt P50406
Pathways JAK-STAT Signalweg

Anwendungsinformationen

Produktdetails anti-Serotonin Receptor 6 Antikörper Target Details Serotonin Receptor 6 Handhabung Bilder zurück nach oben
Applikations-hinweise Approved: IHC, IHC-P (2.5 - 5 μg/mL)
Kommentare

Target Species of Antibody: Human

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Serotonin Receptor 6 Antikörper Target Details Serotonin Receptor 6 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-Serotonin Receptor 6 Antikörper Target Details Serotonin Receptor 6 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Serotonin Receptor 6 (HTR6) (C-Term) antibody (ABIN1048967) Anti-HTR6 / 5-HT6 antibody ABIN1048967 IHC staining of human brain, neurons and glia...
Immunohistochemistry (IHC) image for anti-Serotonin Receptor 6 (HTR6) (C-Term) antibody (ABIN1048967) Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)