CD45 Antikörper (AA 535-730)

Produktnummer ABIN967867

Produktdetails anti-CD45 Antikörper

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Bindungsspezifität: AA 535-730

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD45 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Kreuzreaktivität: Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CD45 aa. 535-730 (alternatively spliced)

Klon: 69-CD45

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-CD45 Antikörper (ABIN967867)

Wu, Fu, Shen: "SKAP55 coupled with CD45 positively regulates T-cell receptor-mediated gene transcription." in: Molecular and cellular biology, Vol. 22, Issue 8, pp. 2673-86, (2002) (PubMed).

Wang, Liang, Esselman: "Regulation of the calcium/NF-AT T cell activation pathway by the D2 domain of CD45." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 5, pp. 2557-64, (2000) (PubMed).

Phipps, Yousefi, Branch: "Increased enzymatic activity of the T-cell antigen receptor-associated fyn protein tyrosine kinase in asymptomatic patients infected with the human immunodeficiency virus." in: Blood, Vol. 90, Issue 9, pp. 3603-12, (1997) (PubMed).

Biffen, McMichael-Phillips, Larson, Venkitaraman, Alexander: "The CD45 tyrosine phosphatase regulates specific pools of antigen receptor-associated p59fyn and CD4-associated p56lck tyrosine in human T-cells." in: The EMBO journal, Vol. 13, Issue 8, pp. 1920-9, (1994) (PubMed).

Sieh, Bolen, Weiss: "CD45 specifically modulates binding of Lck to a phosphopeptide encompassing the negative regulatory tyrosine of Lck." in: The EMBO journal, Vol. 12, Issue 1, pp. 315-21, (1993) (PubMed).

Details zu CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Andere Bezeichnung: CD45 (Ly-5) (PTPRC Produkte)

Synonyme: PTPRC antikoerper, si:dkeyp-47c5.1 antikoerper, B220 antikoerper, CD45 antikoerper, CD45R antikoerper, GP180 antikoerper, L-CA antikoerper, LCA antikoerper, LY5 antikoerper, T200 antikoerper, Cd45 antikoerper, Ly-5 antikoerper, Lyt-4 antikoerper, loc antikoerper, Lca antikoerper, RT7 antikoerper, cd45 antikoerper, protein tyrosine phosphatase, receptor type C antikoerper, protein tyrosine phosphatase, receptor type, C antikoerper, receptor-type tyrosine-protein phosphatase C antikoerper, PTPRC antikoerper, ptprc antikoerper, LOC100074271 antikoerper, Ptprc antikoerper

Hintergrund: CD45 (also known as leukocyte common antigen, GP180, or T200) is found on all hematopoietic cells except those of red cell origin. The N-terminal domain is a large glycosylated extracellular region of variable length (390-542 amino acids) derived by the alternative splicing of at least three exons (4, 5, or 6). This variation accounts for the different isoforms of CD45 (180-220 kDa). The remainder of the molecule includes a short transmembrane region followed by a large highly conserved cytoplasmic domain of 702 residues. The CD45 cytoplasmic domain possesses an intrinsic protein tyrosine phosphatase (PTPase) activity. This region contains two PTPase domains (domains I and II) which share 40% homology. Both domains are required for optimal PTPase activity and show significant homology with other receptor-like and cytoplasmic PTPases. CD45 plays a critical role in antigen receptor-induced responses of T and B cells. In B cells, CD45 can exist as part of a larger multisubunit complex composed of several tyrosine phosphorylated proteins, including the Src-family PTK, Lyn, or it can exist as the membrane immunoglobulin-associated MB-1/B29 heterodimer. In T cells, CD45 associates with and dephosphorylates both lck and fyn. In addition, CD45 can associate with membrane proteins involved in T cell activation, such as CD2, Thy-1, TCR, and CD26.
Synonyms: Leukocyte Common Antigen, GP180, T200

Molekulargewicht: 180-220 kDa

Pathways: T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling