CD45 Antikörper

Produktnummer ABIN967473

Produktdetails anti-CD45 Antikörper

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD45 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Low-Density Cells from AO/G Rat Peyer's Patches

Klon: HIS24

Isotyp: IgG2b kappa

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CD45 Antikörper (ABIN967473)

Hermans, Deenen, De Boer, Bo, Kroese, Opstelten: "Expression of HIS50 Ag: a rat homologue of mouse heat-stable antigen and human CD24 on B lymphoid cells in the rat." in: Immunology, Vol. 90, Issue 1, pp. 14-22, (1997) (PubMed).

Kroese, Butcher, Lalor, Stall, Herzenberg: "The rat B cell system: the anatomical localization of flow cytometry-defined B cell subpopulations." in: European journal of immunology, Vol. 20, Issue 7, pp. 1527-34, (1990) (PubMed).

Kroese, Wubbena, Opstelten, Deenen, Schwander, De Leij, Vos, Poppema, Volberda, Nieuwenhuis: "B lymphocyte differentiation in the rat: production and characterization of monoclonal antibodies to B lineage-associated antigens." in: European journal of immunology, Vol. 17, Issue 7, pp. 921-8, (1987) (PubMed).

Kroese, Opstelten, Wubbena, Deenen, Aten, Schwander, de Leij, Nieuwenhuis: "Monoclonal antibodies to rat B lymphocyte (sub-)populations." in: Advances in experimental medicine and biology, Vol. 186, pp. 81-9, (1985) (PubMed).

Details zu CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Andere Bezeichnung: CD45R (PTPRC Produkte)

Synonyme: PTPRC antikoerper, si:dkeyp-47c5.1 antikoerper, B220 antikoerper, CD45 antikoerper, CD45R antikoerper, GP180 antikoerper, L-CA antikoerper, LCA antikoerper, LY5 antikoerper, T200 antikoerper, Cd45 antikoerper, Ly-5 antikoerper, Lyt-4 antikoerper, loc antikoerper, Lca antikoerper, RT7 antikoerper, cd45 antikoerper, protein tyrosine phosphatase, receptor type C antikoerper, protein tyrosine phosphatase, receptor type, C antikoerper, receptor-type tyrosine-protein phosphatase C antikoerper, PTPRC antikoerper, ptprc antikoerper, LOC100074271 antikoerper, Ptprc antikoerper

Hintergrund: The HIS24 antibody reacts with a developmentally regulated form of CD45 found on most B lymphocytes, including developing B cells in the bone marrow and peripheral B cells, but not plasma cells. The level of expression of this CD45R antigen appears to be an indicator of both maturational stages in the B-cell lineage and of functionally distinct B-cell subsets. The HIS24 mAb, in combination with other markers such as TdT and Ig expression, is effective in the identification of B-cell progenitors. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the rat are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.

Pathways: T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling