alpha Tubulin Antikörper (AA 427-441) (HRP)
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- Target Alle alpha Tubulin (TUBA1) Antikörper anzeigen
- alpha Tubulin (TUBA1)
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Bindungsspezifität
- AA 427-441
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Reaktivität
- Human, Maus, Ratte, Huhn, Rind (Kuh), Ziege, Schaf
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser alpha Tubulin Antikörper ist konjugiert mit HRP
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Applikation
- Western Blotting (WB), ELISA
- Spezifität
- Anti-Tubulin is directed against human alpha Tubulin protein. The Tubulin Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
- Kreuzreaktivität
- Maus, Ratte (Rattus), Huhn, Schaf, Ziege
- Produktmerkmale
- Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.
- Sterilität
- Sterile filtered
- Immunogen
- Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 427-441 of Human alpha Tubulin.
- Isotyp
- IgG
- Top Product
- Discover our top product TUBA1 Primärantikörper
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- Applikationshinweise
- Anti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract.
- Kommentare
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Gene Name: TUBA1B
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
- Konzentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Konservierungsmittel
- Gentamicin sulfate
- Handhabung
- Do NOT add Sodium Azide!
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening.
- Haltbarkeit
- 12 months
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- Target
- alpha Tubulin (TUBA1)
- Andere Bezeichnung
- Alpha-Tubulin (TUBA1 Produkte)
- Synonyme
- Tuba1 antikoerper, K-ALPHA-1 antikoerper, Tuba-1 antikoerper, cb944 antikoerper, fb22g06 antikoerper, tuba1 antikoerper, wu:fb22g06 antikoerper, Calpha1 antikoerper, tubulin, alpha 1A antikoerper, tubulin alpha 1b antikoerper, tubulin, alpha 1b antikoerper, tubulin, alpha 1B antikoerper, Tuba1a antikoerper, TUBA1B antikoerper, tuba1b antikoerper, Tuba1b antikoerper
- Hintergrund
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Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.
Synonyms: HRP, Loading Control Antibody - Pathways
- Microtubule Dynamics
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