CTDSP1 Antikörper (Internal Region)

Produktnummer ABIN951724

Produktdetails anti-CTDSP1 Antikörper

Target: CTDSP1 (CTD (Carboxy-terminal Domain, RNA Polymerase II, Polypeptide A) Small Phosphatase 1 (CTDSP1))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CTDSP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: EAKAQDSDKI C

Spezifität: This antibody is expected to recognise both reported isoforms (NP_067021 and NP_872580).

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Dog, Cow.
Species reactivity (tested):Human, Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_067021.1, NP_872580.1. Genename: CTDSP1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CTDSP1

Target: CTDSP1 (CTD (Carboxy-terminal Domain, RNA Polymerase II, Polypeptide A) Small Phosphatase 1 (CTDSP1))

Andere Bezeichnung: CTDSP1 (CTDSP1 Produkte)

Synonyme: CTDSP1 antikoerper, NIF3 antikoerper, NLI-IF antikoerper, NLIIF antikoerper, SCP1 antikoerper, fj83f12 antikoerper, im:7141428 antikoerper, wu:fj83f12 antikoerper, zgc:113169 antikoerper, GIP antikoerper, Nif3 antikoerper, CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1 antikoerper, CTD small phosphatase 1 antikoerper, carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 antikoerper, CTDSP1 antikoerper, Ctdsp1 antikoerper, ctdsp1 antikoerper, HCAG_04820 antikoerper, CTRG_06220 antikoerper

Hintergrund: CTDSP1 is a class 2C phosphatase with activity dependent on the conserved DxD motif. Expression of CTDSP1 inhibited activated transcription from several promoter-reporter gene constructs, but expression of a mutant lacking phosphatase activity enhanced transcription. Neuronal gene transcription is repressed in nonneuronal cells by the repressor element-1 (RE1)-silencing transcription factor/neuron-restrictive silencer factor (REST/NRSF, 600571) complex. REST/NRSF recruits SCPs to neuronal genes that contain RE1 elements, leading to neuronal gene silencing in nonneuronal cells. Phosphatase-inactive forms of SCP interfere with REST/NRSF function and promote neuronal differentiation of P19 stem cells. Likewise, small interfering RNA directed to the single Drosophila SCP unmasks neuronal gene expression in S2 cells. Thus, SCP activity is an evolutionarily conserved transcriptional regulator that acts globally to silence neuronal genes.Synonyms: Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1, NIF3, NLI-IF, NLI-interacting factor 3, NLIIF, SCP1, Small C-terminal domain phosphatase 1

Gen-ID: 58190

NCBI Accession: NP_001193807