GFI1B Antikörper (AA 322-326)

Produktnummer ABIN871764

Produktdetails anti-GFI1B Antikörper

Target: GFI1B (Growth Factor Independent 1B Transcription Repressor (GFI1B))

Bindungsspezifität: AA 322-326

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFI1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: The antibody detects endogenous level of total GFI1b protein.

Aufreinigung: Affinity purified

Immunogen: Peptide sequence around AA 322-326 (H-R-E-S-Q) derived from Human GFI1b. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000  

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Details zu GFI1B

Target: GFI1B (Growth Factor Independent 1B Transcription Repressor (GFI1B))

Andere Bezeichnung: GFI1b  (GFI1B Produkte)

Synonyme: GFI1B antikoerper, GFI antikoerper, znf544 antikoerper, MGC80648 antikoerper, Gfi-1B antikoerper, growth factor independent 1B transcriptional repressor antikoerper, growth factor independent 1B transcription repressor L homeolog antikoerper, Zinc finger protein Gfi-1b antikoerper, growth factor independent 1B antikoerper, GFI1B antikoerper, Gfi1b antikoerper, gfi1b.L antikoerper, gfi1b antikoerper

Hintergrund: Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Transcriptional repressor or activator depending on both promoter and cell type context, represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1, GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription, as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.

Molekulargewicht: 38 kDa

Gen-ID: 8328

NCBI Accession: NP_001128

UniProt: Q5VTD9

Pathways: Cellular Response to Molecule of Bacterial Origin