F4/80 Antikörper (FITC)

Produktnummer ABIN784615

Produktdetails anti-F4/80 Antikörper (FITC)

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reaktivität: Maus, Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser F4/80 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Spezifität: This antibody recognizes the F4/80 antigen on major subpopulations of resident tissue macrophages (Schaller et al. 2002). Antigen Distribution Isolated Cells: The antigen is expressed in vitro on over 80 % of M-CSF stimulated bone marrow derived macrophages, after a few days of culture. It is absent from granulocytes, lymphocytes and thrombocytes. Tissue Sections: The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin) except lung. It is also present on Langerhans cells in the skin and Kupffer cells in the liver. In complete Freund's adjuvant induced granulomas, the antigen is expressed by inflammatory macrophages, but is absent from epitheloid cells.Isolated Cells:Tissue Sections:

Kreuzreaktivität (Details): Species reactivity (tested):Mouse (major subpopulation of resident tissue macrophages), Human (heart macrophages)

Aufreinigung: Affinity chromatography

Immunogen: Cultured macrophages

Klon: BM8

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide, 10 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu F4/80

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Andere Bezeichnung: Macrophage F4/80 Antigen (EMR1 Produkte)

Synonyme: EMR1 antikoerper, TM7LN3 antikoerper, DD7A5-7 antikoerper, EGF-TM7 antikoerper, F4/80 antikoerper, Gpf480 antikoerper, Ly71 antikoerper, adhesion G protein-coupled receptor E1 antikoerper, ADGRE1 antikoerper, LOC100424335 antikoerper, Adgre1 antikoerper

Hintergrund: The antigen expression increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas and has been shown to be a unique histological marker of the progression from peri-insulitis to β-cell destruction and diabetes in a mouse diabetes model.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480

Gen-ID: 13733

NCBI Accession: NP_034260

UniProt: Q61549