F4/80 Antikörper (FITC)

Produktnummer ABIN2191820

Produktdetails anti-F4/80 Antikörper (FITC)

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser F4/80 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität (Details): Cross reactivity: Mouse granulocytes : No, Mouse mast cells : No, Mouse platelets : No, Mouse lymphocytes : No, Mouse fibroblasts : No, Mouse endothelial cells : No

Sterilität: 0.2 μm filtered

Klon: BM8

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Mouse macrophages control Negative Mouse fibroblasts or granulocytes control

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 1.0 % bovine serum albumin and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen für anti-F4/80 Antikörper (FITC) (ABIN2191820)

Schaller, Macfarlane, Rupec, Gordon, McKnight, Pfeffer: "Inactivation of the F4/80 glycoprotein in the mouse germ line." in: Molecular and cellular biology, Vol. 22, Issue 22, pp. 8035-43, (2002) (PubMed).

Mackler, Iezza, Akin, McMillan, Yellon: "Macrophage trafficking in the uterus and cervix precedes parturition in the mouse." in: Biology of reproduction, Vol. 61, Issue 4, pp. 879-83, (1999) (PubMed).

Leenen, de Bruijn, Voerman, Campbell, van Ewijk: "Markers of mouse macrophage development detected by monoclonal antibodies." in: Journal of immunological methods, Vol. 174, Issue 1-2, pp. 5-19, (1994) (PubMed).

Details zu F4/80

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Abstract: EMR1 Produkte

Synonyme: EMR1 antikoerper, TM7LN3 antikoerper, DD7A5-7 antikoerper, EGF-TM7 antikoerper, F4/80 antikoerper, Gpf480 antikoerper, Ly71 antikoerper, adhesion G protein-coupled receptor E1 antikoerper, ADGRE1 antikoerper, LOC100424335 antikoerper, Adgre1 antikoerper

Hintergrund: The monoclonal antibody BM8 recognizes a 125 kDa extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This murine F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 Molecule shares overall structural homology to other members of the epidermal growth factor (EGF)-TM7 family. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and thrombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. The monoclonal antibody BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model. Immunogen BALB/c macrophages obtained from 14-day-old bone marrow cell cultures