CD45 Antikörper
-
- Target Alle CD45 (PTPRC) Antikörper anzeigen
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
-
Reaktivität
- Human, Non-Human Primate
-
Wirt
- Maus
-
Klonalität
- Monoklonal
-
Konjugat
- Dieser CD45 Antikörper ist unkonjugiert
-
Applikation
- Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
- Spezifität
- The mouse monoclonal antibody HI30 recognizes an extracellular epitope on all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
- Kreuzreaktivität (Details)
- Human, Non-Human Primates
- Aufreinigung
- Purified by protein-G affinity chromatography.
- Reinheit
- > 95 % (by SDS-PAGE)
- Immunogen
- human peripheral blood leukocytes
- Klon
- HI30
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product PTPRC Primärantikörper
-
-
- Applikationshinweise
-
Western blotting: Recommended dilution: 1 μg/mL, non-reducing conditions, band around 200-250 kDa, positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate, non-reducing conditions, 6 % separating gel.
Flow cytometry: Recommended dilution: 1-4 μg/mL - Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Konzentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Do not freeze.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store at 2-8°C. Do not freeze.
-
-
Oncogene MYCN regulates localization of NKT cells to the site of disease in neuroblastoma." in: The Journal of clinical investigation, Vol. 117, Issue 9, pp. 2702-12, (2007) (PubMed).
: "Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function." in: European heart journal, Vol. 26, Issue 12, pp. 1196-204, (2005) (PubMed).
: "CD45 controls interleukin-4-mediated IgE class switch recombination in human B cells through its function as a Janus kinase phosphatase." in: The Journal of biological chemistry, Vol. 277, Issue 32, pp. 28830-5, (2002) (PubMed).
: "
-
Oncogene MYCN regulates localization of NKT cells to the site of disease in neuroblastoma." in: The Journal of clinical investigation, Vol. 117, Issue 9, pp. 2702-12, (2007) (PubMed).
-
- Target
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
- Andere Bezeichnung
- CD45 (PTPRC Produkte)
- Synonyme
- PTPRC antikoerper, si:dkeyp-47c5.1 antikoerper, B220 antikoerper, CD45 antikoerper, CD45R antikoerper, GP180 antikoerper, L-CA antikoerper, LCA antikoerper, LY5 antikoerper, T200 antikoerper, Cd45 antikoerper, Ly-5 antikoerper, Lyt-4 antikoerper, loc antikoerper, Lca antikoerper, RT7 antikoerper, cd45 antikoerper, protein tyrosine phosphatase, receptor type C antikoerper, protein tyrosine phosphatase, receptor type, C antikoerper, receptor-type tyrosine-protein phosphatase C antikoerper, PTPRC antikoerper, ptprc antikoerper, LOC100074271 antikoerper, Ptprc antikoerper
- Hintergrund
- Protein tyrosine phosphatase receptor type C,CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10 % of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.,LCA, T200, LY5, B220, GP180, TPC
- Gen-ID
- 5788
- UniProt
- P08575
- Pathways
- T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
-