Osteoactivin (AA 85-135) Antikörper

Details zu Produkt Nr. ABIN749093, Anbieter: Anmelden zum Anzeigen
Antigen
  • HGFIN
  • NMB
  • DC-HIL
  • Dchil
  • ipd
  • glycoprotein nmb
  • glycoprotein (transmembrane) nmb
  • glycoprotein nmb L homeolog
  • Gpnmb
  • GPNMB
  • gpnmb.L
Epitop
AA 85-135
20
18
14
11
9
8
8
7
5
4
4
3
2
2
2
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
120
27
23
5
5
5
4
4
2
2
2
1
1
Wirt
Kaninchen
66
59
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
5
5
4
4
4
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
77
60
38
20
14
12
4
3
3
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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'Independent Validation' Siegel
Antigen Osteoactivin
Validierte Anwendung Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)
Positivkontrolle Tumor cell transplanted mouse brain was dissected
Bewertung The staining pattern was as expected for Osteoactivin as membrane protein.
Primärantikörper ABIN749093
Isotypkontrolle rabbit IgG
Sekundärantikörper Donkey-anti-Rabbit-Cy3
Protokoll
  • Tumor cell transplanted mouse brain was dissected, fixed in 4% PFA for overnight, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
  • Immunostaining for Cryo Sections:
    • Dry slides at 50-55°C for 15-30 min (or RT for 30min)
    • Wash three times with PBS 5min (shake gently)
    • Block with 10% Normal Donkey Serum (NDS) at RM for 1hour
    • Incubate with primary antibody at 4°C overnight (Prepared in 2% NDS)
    • Rabbit-anti-Osteoactivin antibody (ABIN749093) diluted 1:50-1:400
    • Wash three times with PBS 5min (shake gently)
    • Incubate with secondary antibody together with DAPI at RT for 1h (Prepared in 2% NDS), cover the chamber with foil
    • Donkey-anti-Rabbit-Cy3
    • DAPI 1:100
    • Wash three times with PBS 5min (shake gently)
    • Add three drops of water-based Immu-mount
    • Cover with 24x60 cover glass
  • Imaging
    • Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.
    Anmerkungen ABIN749093 worked within the diution range 1:50 - 1:200; the antibody did not work well at a 1:400 dilution.
    Validierungsbilder
    Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) validation image for anti-Osteoactivin (AA 85-135) antibody (ABIN749093) IF validation of osteoactivin antibody ABIN749093 on mouse brain tumor sections. A. A...
    Immunogen KLH conjugated synthetic peptide derived from human GPNMB
    Isotyp IgG
    Reinigung Purified by Protein A.
    Andere Bezeichnung Gpnmb
    Hintergrund

    The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

    Subcellular location: Extracellular

    Synonyms: NMB, HGFIN, Transmembrane glycoprotein NMB, Transmembrane glycoprotein HGFIN, GPNMB, UNQ1725/PRO9925
    Gen-ID 10457
    UniProt Q14956
    Applikationshinweise WB 1:100-1000
    IHC-P 1:100-500
    IF(IHC-P) 1:50-200
    Beschränkungen Nur für Forschungszwecke einsetzbar
    Format Liquid
    Konzentration 1 μg/μL
    Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
    Konservierungsmittel Sodium azide
    Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung -20 °C
    Informationen zur Lagerung Store at -20°C
    Haltbarkeit 12 months
    Bilder des Herstellers
    SDS-PAGE (SDS) image for anti-Osteoactivin (AA 85-135) antibody (ABIN749093) Formalin-fixed and paraffin embedded rat lung labeled with Anti-GPNMB/Osteoactivin Po...
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Osteoactivin (AA 85-135) antibody (ABIN749093) Formalin-fixed and paraffin embedded rat lung labeled with Anti-GPNMB/Osteoactivin Po...
    Produkt verwendet in: Arosarena, Dela Cadena, Denny, Bryant, Barr, Thorpe, Safadi: "Osteoactivin Promotes Migration of Oral Squamous Cell Carcinomas." in: Journal of cellular physiology, Vol. 231, Issue 8, pp. 1761-70, 2016 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

    Frara, Abdelmagid, Tytell, Amin, Popoff, Safadi, Barbe: "Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping." in: BMC musculoskeletal disorders, Vol. 17, pp. 34, 2016 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB), Western Blotting (WB) (Probematerial (Species): Rat (Rattus)).

    Ruan, Li, Nie, Zhou, Zou: "Differential expression of glycoprotein non-metastatic melanoma protein B (GPNMB) involved in trichostatin A-induced apoptosis in gastric cancer." in: International journal of clinical and experimental medicine, Vol. 7, Issue 12, pp. 4857-66, 2015 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Human).

    Abdelmagid, Sondag, Moussa, Belcher, Yu, Stinnett, Novak, Mbimba, Khol, Hankenson, Malcuit, Safadi: "Mutation in Osteoactivin Promotes Receptor Activator of NF?B Ligand (RANKL)-mediated Osteoclast Differentiation and Survival but Inhibits Osteoclast Function." in: The Journal of biological chemistry, Vol. 290, Issue 33, pp. 20128-46, 2015 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Mouse (Murine)).

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