POLD3 Antikörper (AA 203-275)

Produktnummer ABIN7150235

Produktdetails anti-POLD3 Antikörper

Target: POLD3 (Polymerase (DNA-Directed), delta 3, Accessory Subunit (POLD3))

Bindungsspezifität: AA 203-275

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Kreuzreaktivität: Human, Maus

Aufreinigung: >95%, Protein G purified

Immunogen: Recombinant Human DNA polymerase delta subunit 3 protein (203-275AA)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:300, IF:1:50-1:200,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu POLD3

Target: POLD3 (Polymerase (DNA-Directed), delta 3, Accessory Subunit (POLD3))

Andere Bezeichnung: POLD3 (POLD3 Produkte)

Synonyme: zgc:136471 antikoerper, P66 antikoerper, P68 antikoerper, 2410142G14Rik antikoerper, C85233 antikoerper, polymerase (DNA-directed), delta 3, accessory subunit L homeolog antikoerper, polymerase (DNA-directed), delta 3, accessory subunit antikoerper, DNA polymerase delta 3, accessory subunit antikoerper, pold3.L antikoerper, pold3 antikoerper, POLD3 antikoerper, Pold3 antikoerper

Hintergrund:

Background: As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA (PubMed:10219083, PubMed:10852724, PubMed:11595739, PubMed:16510448, PubMed:24035200). Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5\\\'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context, POLD3, along with PCNA and RFC1-replication factor C complex, is required to recruit POLD1, the catalytic subunit of the polymerase delta complex, to DNA damage sites (PubMed:20227374). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion (PubMed:19074196, PubMed:25628356, PubMed:27185888). Also involved in TLS, as a component of the POLZ complex. Along with POLD2, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7 (PubMed:24449906).

Aliases: DNA polymerase delta subunit 3 antibody, DNA polymerase delta subunit p66 antibody, DNA polymerase delta, subunit 3 antibody, DNA polymerase subunit delta p66 antibody, DPOD3_HUMAN antibody, KIAA0039 antibody, P66 antibody, P68 antibody, Pol delta C subunit (p66) antibody, Pold3 antibody, Polymerase (DNA directed), delta 3 antibody, Polymerase (DNA-directed), delta 3, accessory subunit antibody, PPP1R128 antibody, Protein phosphatase 1, regulatory subunit 128 antibody

UniProt: Q15054

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA