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DYKDDDDK Tag Antikörper

WB, ELISA, IP, IF Wirt: Maus Monoclonal 4G5C8 unconjugated

Immunoprecipitating Flag Tag in 293F transfected whole cell lysate Lane 1: Mouse control IgG instead of in Flag Tag in 293F transfected whole cell lysate. (DYKDDDDK Tag Antikörper)

Western Blot Positive WB detected in: 10 ng Flag Tag fusion protein Flag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa (DYKDDDDK Tag Antikörper)

Western Blot Positive WB detected in: Flag Tag fusion protein at 50 ng, 25 ng, 12. (DYKDDDDK Tag Antikörper)

Immunoprecipitating Flag Tag in Transfected HEK-293 cell whole cell lysate Lane 1: Mouse control IgG instead of in Transfected HEK-293 cell whole cell lysate Lane 2: (1 μL) + Transfected HEK-293 cell whole cell lysate (500 μg) Lane 3: Transfected HEK-293 cell whole cell lysate (20 μg) For western blotting, the blot was detected with at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 (DYKDDDDK Tag Antikörper)

Western Blot Positive WB detected in: Flag Tag fusion protein1, 2, 3, 4 at 50 ng All lanes: Flag Tag antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 59. (DYKDDDDK Tag Antikörper)

Immunofluorescence staining of 293T cells transfected with Flag tag by at 1:300, counter-stained with DAPI. (DYKDDDDK Tag Antikörper)

6 Abbildungen

Produktnummer ABIN7322021

Datenblatt (PDF)

Target Alle DYKDDDDK Tag Produkte

DYKDDDDK Tag

Reaktivität

Bitte anfragen
Wirt
57

51

9

8

6
Maus
Passende Sekundärantikörper
Klonalität
66

64
Monoklonal
Konjugat
46

15

10

7

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser DYKDDDDK Tag Antikörper ist unkonjugiert
Applikation
78

61

28

23

20

19

12

12

10

6

5

4

3

3

3

2

2

2

2

1

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Aufreinigung

Protein G purified

Immunogen

DYKDDDDK(Flag) synthetic peptide conjugate to KLH

Klon

4G5C8

Isotyp

IgG2a
anti-DYKDDDDK Tag antibody
WB, ELISA, IP Wirt: Kaninchen Polyclonal unconjugated

anti-DYKDDDDK Tag antibody
WB, IP, IF Wirt: Maus Monoclonal 2C5 unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IF, FACS Wirt: Kaninchen Polyclonal unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IP, DB, ISt Wirt: Maus Monoclonal FG4R unconjugated

anti-DYKDDDDK Tag antibody
D Wirt: Maus Monoclonal 29E4-G7 unconjugated

anti-DYKDDDDK Tag antibody
Reaktivität: Human WB Wirt: Kaninchen Polyclonal RB0493 unconjugated

anti-DYKDDDDK Tag antibody (HRP)
WB, ELISA, IP Wirt: Kaninchen Polyclonal HRP

anti-DYKDDDDK Tag antibody
IP Wirt: Maus Monoclonal 29E4-G7 unconjugated

anti-DYKDDDDK Tag antibody
WB, IP, IF Wirt: Maus unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IP Wirt: Maus Monoclonal 5A8E5 unconjugated

Applikationshinweise

Recommended dilution: WB:1:1000-1:10000, IF:1:100-1:300, IP:0.5 μL-2 μL,

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C,-80 °C

Informationen zur Lagerung

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

DYKDDDDK Tag

Andere Bezeichnung

Flag Tag (DYKDDDDK Tag Produkte)

Substanzklasse

Tag

Hintergrund

Background: Flag-tag, or Flag octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
Aliases: DDK tag, DYKDDDDK, Flag, Flag Tag

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