DYKDDDDK Tag Antikörper
Kurzübersicht für DYKDDDDK Tag Antikörper (ABIN387849)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Sequenz
- DYKDDDDK
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Aufreinigung
- This antibody is purified through a protein A column, followed by peptide affinity purification.
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Immunogen
- KLH conjugated synthetic peptide (CDYKDDDDKDYKDDDDK) was used as antigen.
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Isotyp
- IgG
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Applikationshinweise
- WB: 1:2000. WB: 1:2000
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Buffer
- Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
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Haltbarkeit
- 6 months
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: "Chromatin-remodelling factor Brg1 regulates myocardial proliferation and regeneration in zebrafish." in: Nature communications, Vol. 7, pp. 13787, (2018) (PubMed).
: "Sensitive and Quantitative Three-Color Protein Imaging in Fission Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with Experimentally-Characterized In Vivo Maturation Kinetics." in: PLoS ONE, Vol. 11, Issue 8, pp. e0159292, (2017) (PubMed).
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- DYKDDDDK Tag
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Substanzklasse
- Tag
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Hintergrund
- Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1). Abgent's DYKDDDDK antibody binds to the same epitope recognized by Sigma's Anti-FLAG antibodies. (FLAG is a registered trademark of Sigma-Aldrich Co., which is not affiliated with Abgent).
Target
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