DYKDDDDK Tag Antikörper

Details zu Produkt Nr. ABIN387700, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
223
119
65
18
18
10
9
7
5
3
2
1
1
1
Wirt
Maus
189
129
34
30
16
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
40
22
18
9
9
9
9
9
8
8
7
7
7
7
7
6
5
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
323
128
120
101
76
58
45
14
11
8
7
6
4
4
4
3
3
3
3
2
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Marke THE™
Immunogen A synthetic peptide (DYKDDDDK) coupled - KLH
Sequenz DYKDDDDK
Klon 5A8E5
Isotyp IgG
Spezifität THETM DYKDDDDK Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal and internal tagged fusion proteins.
Produktmerkmale Mouse Anti-DYKDDDDK-tag Monoclonal Antibody is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins.
Reinigung Protein A affinity column
Substanzklasse Tag
Hintergrund The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors.
Applikationshinweise Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Immunoprecipitation: 1 µg/mL
Other applications: user-optimized
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Avoid repeated freezing and thawing cycles.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
Bilder des Herstellers
 image for anti-DYKDDDDK Tag antibody (ABIN387700) Loading: Lane 1.N-terminal DYKDDDDK-fusion protein (400 ng) Lane 2.C-terminal DYKDDDD...
 image for anti-DYKDDDDK Tag antibody (ABIN387700) Loading: Lane 1-3 Multiple Tag (Purified) (ABIN1536315, 400 ng, 80 ng,16 ng) Lane 4. ...
 image for anti-DYKDDDDK Tag antibody (ABIN387700) Lane1: 1 µL E-coli lysate with DYKDDDDK-tag protein (MW: 57 kDa) and 2 µg Mouse Anti-...
Produkt verwendet in: Reuben, Phénix, Santos, Lapointe: "The WT hemochromatosis protein HFE inhibits CD8? T-lymphocyte activation." in: European journal of immunology, Vol. 44, Issue 6, pp. 1604-14, 2014 (PubMed).

Maryon, Molloy, Kaplan: "Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1." in: American journal of physiology. Cell physiology, Vol. 304, Issue 8, pp. C768-79, 2013 (PubMed).

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