DYKDDDDK Tag Antikörper

Produktnummer ABIN387700

Dieser Maus Monoklonal Antikörper detektiert spezifisch DYKDDDDK Tag in WB, ELISA und IP. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für DYKDDDDK Tag Antikörper (ABIN387700)

Target: DYKDDDDK Tag

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DYKDDDDK Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Klon: 5A8E5

Produktdetails anti-DYKDDDDK Tag Antikörper

Sequenz: DYKDDDDK

Spezifität: THE^TM DYKDDDDK Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal and internal tagged fusion proteins.

Produktmerkmale: Mouse Anti-DYKDDDDK-tag Monoclonal Antibody is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins.

Aufreinigung: Protein A affinity column

Immunogen: A synthetic peptide (DYKDDDDK) coupled - KLH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Immunoprecipitation: 1 µg/mL
Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

Referenzen für anti-DYKDDDDK Tag Antikörper (ABIN387700)

Reuben, Phénix, Santos, Lapointe: "The WT hemochromatosis protein HFE inhibits CD8? T-lymphocyte activation." in: European journal of immunology, Vol. 44, Issue 6, pp. 1604-14, (2014) (PubMed).

Maryon, Molloy, Kaplan: "Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1." in: American journal of physiology. Cell physiology, Vol. 304, Issue 8, pp. C768-79, (2013) (PubMed).

Details zu DYKDDDDK Tag

Target: DYKDDDDK Tag

Substanzklasse: Tag

Hintergrund: The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors.