SMURF1 Antikörper (N-Term)

Produktnummer ABIN6992054

Produktdetails anti-SMURF1 Antikörper

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Bindungsspezifität: AA 180-230, N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMURF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: SMURF1 antibody is human, mouse and rat reactive. At least three isoforms of SMURF1 are known to exist this antibody will recognize all three isoforms. SMURF1 is predicted to not cross-react with SMURF2.

Aufreinigung: SMURF1 antibody is affinity chromatography purified via peptide column.

Immunogen: SMURF1 antibody was raised against a 16 amino acid peptide near the amino terminus of human SMURF1. The immunogen is located within amino acids 180 - 230 of SMURF1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: SMURF1 body can be used for detection of SMURF1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: SMURF1 antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: SMURF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu SMURF1

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Andere Bezeichnung: SMURF1 (SMURF1 Produkte)

Synonyme: 4930431E10Rik antikoerper, mKIAA1625 antikoerper, RGD1309707 antikoerper, SMAD specific E3 ubiquitin protein ligase 1 antikoerper, E3 ubiquitin-protein ligase SMURF1 antikoerper, SMAD specific E3 ubiquitin protein ligase 1 L homeolog antikoerper, SMURF1 antikoerper, LOC100540915 antikoerper, smurf1 antikoerper, Smurf1 antikoerper, smurf1.L antikoerper

Hintergrund: SMURF1 (SMAD ubiquitin regulatory factor 1) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF1 negatively regulates osteoblast activity and response to bone morphogenesis protein (BMP) through controlling MEKK2 degradation (3). SMURF1 has recently been shown to play a role in breast cancer cell migration and invasion through the downregulation of RhoA (4).

Molekulargewicht: Predicted: 80, 83 kDa

Observed: 78 kDa

Gen-ID: 57154

NCBI Accession: NP_065162

UniProt: Q9HCE7

Pathways: Regulation of Cell Size