IKK alpha Antikörper (C-Term)

Produktnummer ABIN6990273

Dieses Anti-IKK alpha-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von IKK alpha in WB, ELISA, IF und ICC. Geeignet für Human.

Kurzübersicht für IKK alpha Antikörper (C-Term) (ABIN6990273)

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IKK alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-IKK alpha Antikörper

Bindungsspezifität: C-Term

Spezifität: Antibody has no cross response to IKKb or IKKg.

Aufreinigung: IKK alpha Antibody is affinity chromatography purified via peptide column.

Immunogen: IKK alpha antibody was raised against a 19 amino acid peptide near the carboxy terminus of human IKK alpha. The immunogen is located within the last 50 amino acids of IKK alpha.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IKK alpha can be used for detection of IKK alpha by Western blot at 1 μ,g/mL. An 85 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: IKK alpha Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: IKK alpha antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu IKK alpha

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Andere Bezeichnung: IKK alpha

Hintergrund: IKK alpha Antibody: Nuclear factor kappa B (NF-κ,B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κ,B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-κ,B is associated with Iκ,B proteins in the cell cytoplasm, which inhibit NF-κ,B activity. The long-sought Iκ,B kinase (IKK), which phosphorylates Iκ,B, and mediates Iκ,B degradation and NF-κ,B activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα, and IKKβ,). IKKα, and IKKβ, interact with each other and both are essential for the NF-κ,B activation. IKKα, specifically phosphorylates IkB-alpha. IKKα, is expressed in variety of human tissues.

Molekulargewicht: 85 kDa

Gen-ID: 1147

UniProt: O15111

Pathways: PI3K-Akt Signalweg, NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteine