C-JUN Antikörper (pThr91, pThr93)

Produktnummer ABIN6939837

Dieser Maus Monoklonal Antikörper detektiert spezifisch C-JUN in WB, IHC, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human und Ratte.

Kurzübersicht für C-JUN Antikörper (pThr91, pThr93) (ABIN6939837)

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser C-JUN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: C-J 4C4-1

Produktdetails anti-C-JUN Antikörper

Bindungsspezifität: pThr91, pThr93

Verwendungszweck: Phospho C-Jun (Thr91, Thr93) Antibody

Spezifität: This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Aufreinigung: 200ug/ml of Ab Purified by Protein A.

Immunogen: c-Jun protein phosphorylated at T91 and T93

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HEK293, HeLA or A-431 cells, Human normal lung or bladder tissue.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu C-JUN

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Andere Bezeichnung: JUN

Hintergrund:

Activator protein 1(API), cJun, Enhancer Binding Protein AP1, Jun Activation Domain Binding Protein, Jun proto oncogene, Transcription Factor AP1, V jun avian sarcoma virus 17 oncogene homolog, VV jun sarcoma virus 17 oncogene homolog, V-jun avian sarcoma virus 17 oncogene homolog, vJun Avian Sarcoma Virus 17 Oncogene Homolog

HEK293, HeLA or A-431 cells, Human normal lung or bladder tissue.

Molekulargewicht: 39kDa

Gen-ID: 3725, 696684

UniProt: P05412

Pathways: MAPK Signalweg, RTK Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteine