HIF1A Antikörper

Produktnummer ABIN6937377

Produktdetails anti-HIF1A Antikörper

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HIF1A Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Spezifität: HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex, ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human HIF1 alpha protein

Klon: HIF1A-84

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Cobalt chloride treated HeLa cells. Breast or bladder carcinomas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu HIF1A

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Andere Bezeichnung: HIF1A (HIF1A Produkte)

Synonyme: hif1a antikoerper, AA959795 antikoerper, HIF1alpha antikoerper, MOP1 antikoerper, bHLHe78 antikoerper, HIF-1A antikoerper, HIF-1alpha antikoerper, HIF1 antikoerper, HIF1-ALPHA antikoerper, PASD8 antikoerper, HIF1-alpha antikoerper, hif-1a antikoerper, hypoxia inducible factor 1 alpha antikoerper, hypoxia inducible factor 1, alpha subunit antikoerper, hypoxia inducible factor 1 alpha subunit antikoerper, HIF (Hypoxia Inducible Factor) homologa antikoerper, hif-1a antikoerper, Hif1a antikoerper, HIF1A antikoerper, hif1a antikoerper, hif-1 antikoerper

Hintergrund: ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78 (bHLHe78), Hypoxia inducible factor 1 alpha, Member of PAS superfamily 1 (MOP1), PAS domain-containing protein 8 (PASD8),HIF1 alpha (Hypoxia-Inducible Factor 1-alpha)
Cellular localisation: Nuclear and cytoplasmic

Molekulargewicht: 92-110kDa

Gen-ID: 3091, 597216

UniProt: Q16665

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effekt