HIF1A Antikörper (AA 432-528)

Produktnummer ABIN363203

Produktdetails anti-HIF1A Antikörper

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Bindungsspezifität: AA 432-528

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Affe, Schaf, Frettchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HIF1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Chromatin Immunoprecipitation (ChIP), Gel Shift (GS)

Spezifität: Reacts with human HIF-1 alpha

Kreuzreaktivität: Maus, Rind (Kuh), Kaninchen, Schaf

Kreuzreaktivität (Details): Cross reacts with proteins from monkey, sheep, mouse, rat, rabbit, pig, bovine and ferret.

Aufreinigung: Purified (protein A)

Immunogen: Fusion protein containing amino acids 432-528 of human HIF-1alpha

Klon: H1alpha67

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
IHC1:100-1:300 WB1:500-1:1000 ICC/ IF1:100-1:500 IP1:10

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.5 mg/mL

Buffer: Tris-glycine, 150 mM NaCl pH 7.5, Sodium azide 0.05 %

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C. Do not freeze.

Details zu HIF1A

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Andere Bezeichnung: Hypoxia-Inducible Factor 1-alpha (HIF1A Produkte)

Synonyme: hif1a antikoerper, AA959795 antikoerper, HIF1alpha antikoerper, MOP1 antikoerper, bHLHe78 antikoerper, HIF-1A antikoerper, HIF-1alpha antikoerper, HIF1 antikoerper, HIF1-ALPHA antikoerper, PASD8 antikoerper, HIF1-alpha antikoerper, hif-1a antikoerper, hypoxia inducible factor 1 alpha antikoerper, hypoxia inducible factor 1, alpha subunit antikoerper, hypoxia inducible factor 1 alpha subunit antikoerper, HIF (Hypoxia Inducible Factor) homologa antikoerper, hif-1a antikoerper, Hif1a antikoerper, HIF1A antikoerper, hif1a antikoerper, hif-1 antikoerper

Hintergrund: HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes.

Gen-ID: 3091

UniProt: Q16665

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effekt