ADO Antikörper (AA 49-261)

Produktnummer ABIN7354747

Produktdetails anti-ADO Antikörper

Target: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Bindungsspezifität: AA 49-261

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADO Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Verwendungszweck: Rabbit IgG Polyclonal Anti-Human ADO Antibody DyLight® 550 Conjugated, Flow Validated.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG Polyclonal Anti-Human ADO Antibody DyLight® 550 Conjugated, Flow Validated.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human ADO recombinant protein (Position: E49-E261). Human ADO shares 90.1% amino acid (aa) sequence identity with mouse ADO.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application details: Flow Cytometry|1-3 μg/1x106 cells

Kommentare:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: At 2-8°C for one year. Protect from light. Do not freeze.

Details zu ADO

Target: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Andere Bezeichnung: ADO (ADO Produkte)

Synonyme: C10orf22 antikoerper, Gm237 antikoerper, RGD1308233 antikoerper, wu:fc32g01 antikoerper, wu:fc52c12 antikoerper, zgc:101580 antikoerper, 2-aminoethanethiol dioxygenase antikoerper, 2-aminoethanethiol (cysteamine) dioxygenase antikoerper, 2-aminoethanethiol (cysteamine) dioxygenase a antikoerper, ADO antikoerper, Ado antikoerper, LOC100351598 antikoerper, adoa antikoerper

Hintergrund:

Synonyms: 2-aminoethanethiol dioxygenase, Cysteamine dioxygenase, ADO, C10orf22

Background: Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.