ADO Antikörper

Produktnummer ABIN7241939

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch ADO in ELISA, WB und IHC. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für ADO Antikörper (ABIN7241939)

Target: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADO Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-ADO Antikörper

Produktmerkmale: Polyclonal Antibody

Aufreinigung: Affinity purification

Immunogen: Synthetic peptide of human ADO

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:1000-1:5000, IHC 1:25-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.3 mg/mL

Buffer: PBS with 0.05 % sodium azide and 50 % glycerol, PH7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Details zu ADO

Target: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Andere Bezeichnung: ADO

Hintergrund: Human thiol dioxygenases include cysteine dioxygenase (CDO, MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO, EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. Mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. The demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.

Molekulargewicht: 30 kDa

NCBI Accession: NP_116193

UniProt: Q96SZ5