PI3K p85 alpha/gamma Antikörper (AA 410-490)

Produktnummer ABIN6283949

Produktdetails anti-PI3K p85 alpha/gamma Antikörper

Target: PI3K p85 alpha/gamma (PI3K p85a/g) (Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g))

Bindungsspezifität: AA 410-490

Reaktivität: Human, Maus, Ratte, Affe, Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PI3K p85 alpha/gamma Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: PI 3-kinase p85α/γ is a protein encoded by the PIK3R1 gene which is approximately 83,5 kDa. PI 3-kinase p85α/γ is localised to the cytoplasm, nucleus and cis-Golgi network. It is involved in RET signalling, the IL-2 pathway, downstream signalling of activated FGFR2 and regulation of lipid metabolism. It binds to activated protein-Tyr kinases, through its SH2 domain, and acts as an adapter by mediating the association of the p110 catalytic unit to the plasma membrane. It is also necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. PI 3-kinase p85α/γ isoform 2 is expressed in skeletal muscle and brain. Mutations in the PIK3R1 gene may result in Short syndrome and Pasli disease. STJ95078 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of PI 3-kinase p85α/γ protein.

Spezifität: PI 3-kinase p85α/γ Polyclonal Antibody detects endogenous levels of PI 3-kinase p85α/γ protein.

Produktmerkmale: Rabbit polyclonal to PI 3-kinase p85α/γ.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PI 3-kinase p85/p55 around the non-phosphorylation site of Y458/199.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000

Kommentare:

Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C, and avoid repeat freeze-thaw cycles.

Details zu PI3K p85 alpha/gamma

Target: PI3K p85 alpha/gamma (PI3K p85a/g) (Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g))

Andere Bezeichnung: PI 3-kinase p85alpha/gamma (PI3K p85a/g Produkte)

Molekulargewicht: 83/54 kDa

Gen-ID: 5295

UniProt: P27986