BRCA1 Antikörper (AA 1360-1440)

Produktnummer ABIN6281204

Produktdetails anti-BRCA1 Antikörper

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Bindungsspezifität: AA 1360-1440

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BRCA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: BRCA1 is a protein encoded by the BRCA1 gene which is approximately 207,7 kDa. BRCA1 is localised to the cytoplasm and nucleus. It is involved in DNA double-strand break repair and meiosis. It is a E3 ubiquitin-protein ligasespecifically mediates the formation of Lys-6-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. The BRCA1-BARD1 heterodimer coordinates a range of cellular pathways such as ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 isoform 1 and 3 are widely expressed. Mutations in the BRCA1 gene may result in pancreatic cancer and breast cancer. STJ91884 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of BRCA1 protein.

Spezifität: BRCA1 Polyclonal Antibody detects endogenous levels of BRCA1 protein.

Produktmerkmale: Rabbit polyclonal to BRCA1.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human BRCA1 around the non-phosphorylation site of S1423.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000

Kommentare:

Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C, and avoid repeat freeze-thaw cycles.

Details zu BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Andere Bezeichnung: BRCA1 (BRCA1 Produkte)

Synonyme: BRCAI antikoerper, BRCC1 antikoerper, BROVCA1 antikoerper, IRIS antikoerper, PNCA4 antikoerper, PPP1R53 antikoerper, PSCP antikoerper, RNF53 antikoerper, iris antikoerper, pscp antikoerper, brcai antikoerper, brcc1 antikoerper, rnf53 antikoerper, brca1 antikoerper, BRCA1 antikoerper, LOC100224649 antikoerper, BRCA1, DNA repair associated antikoerper, breast cancer 1 antikoerper, breast cancer 1, early onset antikoerper, breast cancer 1 L homeolog antikoerper, BRCA1 antikoerper, brca1 antikoerper, LOC755086 antikoerper, Brca1 antikoerper, brca1.L antikoerper

Molekulargewicht: 180 kDa

Gen-ID: 672

UniProt: P38398

Pathways: Zellzyklus, DNA Reparatur, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus