Prostate Specific Antigen Antikörper

Produktnummer ABIN6252869

Produktdetails anti-Prostate Specific Antigen Antikörper

Target: Prostate Specific Antigen (PSA)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Prostate Specific Antigen Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Aufreinigung: Protein G affinity chromatography

Immunogen: Prostate specific antigen from human sperm plasma was used as the immunogen for this PSA antibody.

Klon: SPM352

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the PSA antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Andere Bezeichnung: PSA (PSA Produkte)

Synonyme: APS antikoerper, KLK2A1 antikoerper, PSA antikoerper, hK3 antikoerper, KLK3 antikoerper, Klk1c10l2 antikoerper, RSGK-50 antikoerper, RSKG-50 antikoerper, rGK-3 antikoerper, Kal-3 antikoerper, Kal3 antikoerper, Klk3 antikoerper, AAP-S antikoerper, MP100 antikoerper, Psa antikoerper, R74825 antikoerper, goku antikoerper, kallikrein related peptidase 3 antikoerper, kallikrein 3 antikoerper, kallikrein 1-related peptidase C3 antikoerper, kallikrein B, plasma 1 antikoerper, aminopeptidase puromycin sensitive antikoerper, KLK3 antikoerper, Klk1c3 antikoerper, Klkb1 antikoerper, Npepps antikoerper

Hintergrund: Recognizes a single protein of 33-34  kDa, identified as the prostate specific antigen (PSA). This mAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

Gen-ID: 354

UniProt: P07288

Pathways: Komplementsystem