Prostate Specific Antigen Antikörper (PSA)

Details for Product anti-PSA Antibody No. ABIN1543584
Antigen
  • APS
  • KLK2A1
  • PSA
  • hK3
  • KLK3
  • Klk1c10l2
  • RSGK-50
  • RSKG-50
  • rGK-3
  • AAP-S
  • MP100
  • Psa
  • R74825
  • goku
  • kallikrein related peptidase 3
  • kallikrein 3
  • kallikrein 1-related peptidase C3
  • aminopeptidase puromycin sensitive
  • KLK3
  • Klk1c3
  • Npepps
Reaktivität
Human
630
16
9
5
1
1
1
1
Wirt
Maus
467
133
28
7
7
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser Prostate Specific Antigen Antikörper ist unkonjugiert
36
28
20
16
13
13
5
5
5
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Applikation
Dot Blot (DB), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
365
182
126
121
108
82
48
42
32
26
26
25
14
13
7
7
6
6
6
4
3
2
1
1
1
1
Optionen
'Independent Validation' Siegel
Chargennummer FAPS-A5D5-0003
Validierte Anwendung Flow Cytometry
Positivkontrolle LNCaP cells
Negativkontrolle - DU145 cells - Jurkat cells
Bewertung Strong signal above isotype signal was observed in the positive control sample, while staining of similar level to isotype control was observed in both negative control samples.
Primärantikörper
  • Antibody: Prostate Specific Antigen (PSA)
  • Catalog number: ABIN1543584
  • Lot number: FAPS-A5D5-0003
Isotypkontrolle
  • Antibody: mouse IgG1
Sekundärantikörper
  • Antibody: F(ab’)2 goat anti-mouse IgG-Alexa 647
Kontrollen
  • Positive control: LNCaP prostate tumor cell line
  • Negative control: DU145 prostate tumor cell line, Jurkat T cell leukemia cell line
  • Isotype control: cells treated with mouse IgG1 instead of the primary antibody to confirm that primary antibody binding is specific.
Protokoll
  • Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with 0.05% trypsin / 0.025% EDTA.
  • Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
  • An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37°C.
  • Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
  • Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA and 0.1% Tween 20
  • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA.
  • 1 µg of anti-PSA or mouse IgG1 isotype control were added to the cells and incubated for 30 min at room temperature
  • Cells were washed twice with PBS/BSA/Tween 20
  • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA
  • Secondary antibody was added at a final dilution of 1:500 and incubated for 30 min at room temperature
  • Cells were washed twice with PBS/BSA/Tween 20
  • Cells were resuspended in 0.3 mL PBS/BSA
  • Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
Anmerkungen None
Validierungsbilder
Flow Cytometry validation image for anti-Prostate Specific Antigen (PSA) antibody (ABIN1543584) Figure 1: LNCaP cells stained with anti-PSA (blue) or with isotype control (red).
Flow Cytometry validation image for anti-Prostate Specific Antigen (PSA) antibody (ABIN1543584) Figure 2: DU145 cells stained with anti-PSA (blue) or with isotype control (red).
Flow Cytometry validation image for anti-Prostate Specific Antigen (PSA) antibody (ABIN1543584) Figure 3: Jurkat cells stained with anti-PSA (blue) or with isotype control (red).
Immunogen Native PSA Antigen
Klon A5D5-1
Isotyp IgG1
Reinigung Protein A affinity column
Plasmids, Primers & others Plasmide, Primers & weitere Prostate Specific Antigen products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung PSA (PSA Antibody Abstract)
Pathways Komplementsystem
Kommentare

Other Applications have not been tested.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.8 mg/mL
Buffer PBS, pH 7.4, 0.09% sodium azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Haben Sie etwas anderes gesucht?