Prostate Specific Antigen Antikörper

Produktnummer ABIN1543584

Produktdetails anti-Prostate Specific Antigen Antikörper

Target: Prostate Specific Antigen (PSA)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Prostate Specific Antigen Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Dot Blot (DB)

Aufreinigung: Protein A affinity column

Immunogen: Native PSA Antigen

Klon: A5D5-1

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Other Applications have not been tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (FACS)

Validierung #029617 (Flow Cytometry)

by: Flow Cytometry & Cell Separation Facility, Purdue University

No.: #029617

Datum: 22.02.2014

Chargennummer: FAPS-A5D5-0003

Validierte Anwendung: Flow Cytometry

Positivkontrolle: LNCaP cells

Negativkontrolle: - DU145 cells - Jurkat cells

Bewertung: Strong signal above isotype signal was observed in the positive control sample, while staining of similar level to isotype control was observed in both negative control samples.

Validierungsbilder

Figure 1: LNCaP cells stained with anti-PSA (blue) or with isotype control (red).

Figure 2: DU145 cells stained with anti-PSA (blue) or with isotype control (red).

Figure 3: Jurkat cells stained with anti-PSA (blue) or with isotype control (red).

Protokoll

Primärantikörper:

• Antibody: Prostate Specific Antigen (PSA)
• Catalog number: ABIN1543584
• Lot number: FAPS-A5D5-0003

Sekundärantikörper:

• Antibody: F(ab’)2 goat anti-mouse IgG-Alexa 647

Full Protocol:

• Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with 0.05% trypsin / 0.025% EDTA.
• Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
• An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37°C.
• Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
• Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA and 0.1% Tween 20
• Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA.
• 1 µg of anti-PSA or mouse IgG1 isotype control were added to the cells and incubated for 30 min at room temperature
• Cells were washed twice with PBS/BSA/Tween 20
• Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA
• Secondary antibody was added at a final dilution of 1:500 and incubated for 30 min at room temperature
• Cells were washed twice with PBS/BSA/Tween 20
• Cells were resuspended in 0.3 mL PBS/BSA
• Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).

Anmerkungen: None

Handhabung

Format: Liquid

Konzentration: 1.8 mg/mL

Buffer: PBS, pH 7.4, 0.09% sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Details zu Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Andere Bezeichnung: PSA (PSA Produkte)

Synonyme: APS antikoerper, KLK2A1 antikoerper, PSA antikoerper, hK3 antikoerper, KLK3 antikoerper, Klk1c10l2 antikoerper, RSGK-50 antikoerper, RSKG-50 antikoerper, rGK-3 antikoerper, Kal-3 antikoerper, Kal3 antikoerper, Klk3 antikoerper, AAP-S antikoerper, MP100 antikoerper, Psa antikoerper, R74825 antikoerper, goku antikoerper, kallikrein related peptidase 3 antikoerper, kallikrein 3 antikoerper, kallikrein 1-related peptidase C3 antikoerper, kallikrein B, plasma 1 antikoerper, aminopeptidase puromycin sensitive antikoerper, KLK3 antikoerper, Klk1c3 antikoerper, Klkb1 antikoerper, Npepps antikoerper

Pathways: Komplementsystem