PODXL Antikörper (Extracellular Domain)

Produktnummer ABIN6252522

Produktdetails anti-PODXL Antikörper

Target: PODXL (Podocalyxin-Like (PODXL))

Bindungsspezifität: Extracellular Domain

Reaktivität: Human, Ratte, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PODXL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF)

Aufreinigung: Protein G affinity chromatography

Immunogen: A human recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain was used as the immunogen for the Podocalyxin antibody.

Klon: 3D3

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells

IF: 1-2 μg/mL

IP: 1-2 μg/500 μg protein

WB: 0.5-1 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Podocalyxin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Andere Bezeichnung: Podocalyxin (PODXL Produkte)

Synonyme: Gp200 antikoerper, PC antikoerper, PCLP antikoerper, PCLP-1 antikoerper, PCB antikoerper, AW121214 antikoerper, Ly102 antikoerper, Pclp1 antikoerper, podocalyxin antikoerper, MEP21 antikoerper, PODXL antikoerper, Gp135 antikoerper, PCLP1 antikoerper, cPCLP1 antikoerper, podxl antikoerper, fc18d02 antikoerper, wu:fc18d02 antikoerper, Pc antikoerper, Pcb antikoerper, Podxl antikoerper, podocalyxin like antikoerper, pyruvate carboxylase antikoerper, podocalyxin-like antikoerper, PODXL antikoerper, PC antikoerper, Podxl antikoerper, podxl antikoerper, Pcx antikoerper

Hintergrund: Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Gen-ID: 5420

UniProt: O00592

Pathways: Tube Formation