Cyclin B1 Antikörper

Produktnummer ABIN6251960

Produktdetails anti-Cyclin B1 Antikörper

Target: Cyclin B1 (CCNB1)

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin B1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant human full-length protein was used as the immunogen for the Cyclin B1 antibody.

Klon: BCLB1-1

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Titering of the Cyclin B1 antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu Cyclin B1

Target: Cyclin B1 (CCNB1)

Andere Bezeichnung: Cyclin B1 (CCNB1 Produkte)

Synonyme: ccnb antikoerper, cycb antikoerper, cb267 antikoerper, cycb1 antikoerper, wu:fa19g04 antikoerper, wu:fb16d01 antikoerper, wu:fb16e07 antikoerper, wu:fi21c01 antikoerper, ccnb1 antikoerper, MGC53596 antikoerper, CCNB antikoerper, Ccnb1-rs1 antikoerper, Ccnb1-rs13 antikoerper, CycB1 antikoerper, Cycb-4 antikoerper, Cycb-5 antikoerper, Cycb1-rs1 antikoerper, cyclin B1 antikoerper, cyclin B1 S homeolog antikoerper, ccnb1 antikoerper, ccnb1.2 antikoerper, ccnb1.S antikoerper, CCNB1 antikoerper, Ccnb1 antikoerper, ccnb1.2.S antikoerper

Hintergrund: Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis. The gene product complexes with p34 (Cdk1) to form the maturation-promoting factor (MPF). Cyclin B1 contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. Its activation is well-regulated, and positive feedback loops ensure that once the CCNB1-Cdk1 complex is activated, it is not deactivated. CCNB1-Cdk1 is involved in the early events of mitosis, such as chromosome condensation, nuclear envelope breakdown, and spindle pole assembly. Once activated, CCNB1-Cdk1 promotes several of the events of early mitosis. The active complex phosphorylates and activates 13S condensin, which helps to condense chromosomes. Another important function of the complex is to break down the nuclear envelope. The nuclear envelope is a membranous structure containing large protein complexes supported by a network of nuclear lamins. Phosphorylation of the lamins by CCBN1-Cdk1 causes them to dissociate, compromising the structural integrity of the nuclear envelope so that it breaks down. The destruction of the nuclear envelope is important because it allows the mitotic spindle to access the chromosomes. [Wiki]

Gen-ID: 891

UniProt: P14635

Pathways: Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase