PLAP Antikörper

Produktnummer ABIN6251805

Produktdetails anti-PLAP Antikörper

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PLAP Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant full-length human ALPP protein was used as the immunogen for the placental Alkaline Phosphatase antibody.

Klon: ALPP-870

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the placental Alkaline Phosphatase antibody should be determined by the researcher.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Andere Bezeichnung: Placental Alkaline Phosphatase (ALPP Produkte)

Synonyme: ECK0378 antikoerper, JW0374 antikoerper, psiA antikoerper, ALP antikoerper, PALP antikoerper, PLAP antikoerper, PLAP-1 antikoerper, ILC antikoerper, CTACK antikoerper, ESkine antikoerper, Akp2 antikoerper, alp antikoerper, zgc:56672 antikoerper, DDBDRAFT_0205491 antikoerper, DDBDRAFT_0231570 antikoerper, DDB_0205491 antikoerper, DDB_0231570 antikoerper, ALPI antikoerper, Actn2lp antikoerper, Alp antikoerper, bacterial alkaline phosphatase antikoerper, alkaline phosphatase, placental antikoerper, alkaline phosphatase, placental type antikoerper, C-C motif chemokine ligand 27 antikoerper, alkaline phosphatase, liver/bone/kidney antikoerper, alkaline phosphatase antikoerper, alkaline phosphatase, intestinal antikoerper, PDZ and LIM domain 3 antikoerper, phoA antikoerper, ALPP antikoerper, LOC100436725 antikoerper, CCL27 antikoerper, alpl antikoerper, alp antikoerper, ALPI antikoerper, Pdlim3 antikoerper, Alpp antikoerper

Hintergrund: Reacts with a 70  kDa membrane-bound isozyme (Regan and Nagao type) of placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

Gen-ID: 250

UniProt: P05187