Rekombinanter PLAP Antikörper

Produktnummer ABIN6939457

Der Kaninchen Monoklonal anti-PLAP Antikörper wird verwendet zum Nachweis von PLAP in Proben von Human. Er wurde validiert für WB und IHC.

Kurzübersicht für Rekombinanter PLAP Antikörper (ABIN6939457)

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser PLAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: ALPP-2899R

Produktdetails anti-PLAP Antikörper

Verwendungszweck: Recombinant Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Antibody

Spezifität: Reacts with a 70 kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

Aufreinigung: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogen: Recombinant full-length human ALPP protein

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HepG2 cells. Placenta or seminoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Andere Bezeichnung: ALPP

Hintergrund:

Alkaline phosphatase placental type, Alkaline phosphatase Regan Isozyme, ALP, Alp1, ALPP, Germ-cell alkaline phosphatase, nagao Isozyme, PALP, Placental alkaline phosphatase 1, placental heat-stable alkaline phosphatase, PLAP-1, PLAP1

JEG-3 or HepG2 cells. Placenta or seminoma.

Molekulargewicht: 70kDa

Gen-ID: 250, 284255

UniProt: P05187