KIT Antikörper (AA 100-300)

Produktnummer ABIN6190789

Produktdetails anti-KIT Antikörper

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Bindungsspezifität: AA 100-300

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KIT Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Verwendungszweck: Mouse Monoclonal anti-CD117 (KIT/983), Purified, BSA-free

Produktmerkmale: This MAb recognizes a protein of 145 kDa, identified as CD117/p145kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117 has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi?s sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117 is also useful in recognizing myeloblasts in bone marrow biopsy and clot section. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: Recombinant human CD117 fragment around aa100-300

Klon: KIT-983

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 2-4 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Esophagus, Gastrointestinal Stromal Tumor (GIST) or testicular germ cell tumor. Melanocytes in the basal layer of the epidermis and mast cells in the dermis of normal skin.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Konservierungsmittel: Azide free

Details zu KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Andere Bezeichnung: CD117 (KIT Produkte)

Synonyme: C-Kit antikoerper, CD117 antikoerper, PBT antikoerper, SCFR antikoerper, Bs antikoerper, Fdc antikoerper, Gsfsco1 antikoerper, Gsfsco5 antikoerper, Gsfsow3 antikoerper, SCO1 antikoerper, SCO5 antikoerper, SOW3 antikoerper, Ssm antikoerper, Tr-kit antikoerper, W antikoerper, c-KIT antikoerper, c-kit antikoerper, MGF antikoerper, KIT antikoerper, C-KIT antikoerper, kit antikoerper, receptor antikoerper, sparse antikoerper, Xkl-1 antikoerper, kit-a antikoerper, kit-b antikoerper, kl1-A antikoerper, krk1 antikoerper, pbt antikoerper, scfr antikoerper, KIT proto-oncogene receptor tyrosine kinase antikoerper, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a antikoerper, KIT proto-oncogene receptor tyrosine kinase S homeolog antikoerper, KIT antikoerper, Kit antikoerper, kita antikoerper, kit antikoerper, kit.S antikoerper

Molekulargewicht: 145 kDa

Gen-ID: 3815, 479754

UniProt: P10721

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity