CD22 Antikörper (AA 52-178)
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- Target Alle CD22 Antikörper anzeigen
- CD22 (CD22 Molecule (CD22))
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Bindungsspezifität
- AA 52-178
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD22 Antikörper ist unkonjugiert
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Applikation
- Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Coating (Coat)
- Verwendungszweck
- Mouse Monoclonal anti-CD22 / BL-CAM (BLCAM/1795), Purified, with BSA
- Produktmerkmale
- This antibody recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD . It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Aufreinigung
- Purified
- Immunogen
- Recombinant human BL-CAM/CD22 protein (aa52-178) (exact sequence is proprietary)
- Klon
- BLCAM-1795
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product CD22 Primärantikörper
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- Applikationshinweise
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Immunohistology (formalin) 1-2 μg/mL
- ELISA 2-4 μg/mL for coating (order Ab without BSA)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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Raji, Daudi, IM9, JY25 and human peripheral blood lymphocytes. Human Tonsil
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 200 μg/mL
- Buffer
- PBS/0.05 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- CD22 (CD22 Molecule (CD22))
- Andere Bezeichnung
- CD22 / BL-CAM (CD22 Produkte)
- Synonyme
- A530093D23 antikoerper, Lyb-8 antikoerper, Lyb8 antikoerper, SIGLEC-2 antikoerper, SIGLEC2 antikoerper, siglec-2 antikoerper, CD22 antigen antikoerper, CD22 molecule antikoerper, Cd22 antikoerper, CD22 antikoerper
- Hintergrund
- B-lymphocyte cell adhesion molecule (BL-CAM), B-cell receptor CD22, CD22, Lectin 2, Lyb8, Sialic acid-binding Ig-like lectin 2 (Siglec-2), SIGLEC2, T-cell surface antigen Leu-14
- Molekulargewicht
- 130-140 kDa
- Gen-ID
- 933, 579691
- UniProt
- P20273
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