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Rekombinanter CD22 Antikörper (AA 52-178)

CD22 Reaktivität: Human IHC Wirt: Kaninchen Monoclonal MSVA-022R unconjugated Recombinant Antibody
Produktnummer ABIN7477403
  • Target Alle CD22 Antikörper anzeigen
    CD22 (CD22 Molecule (CD22))
    Antikörpertyp
    Recombinant Antibody
    Bindungsspezifität
    • 15
    • 11
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 52-178
    Reaktivität
    • 154
    • 50
    • 24
    • 5
    • 3
    • 2
    • 2
    • 1
    Human
    Wirt
    • 102
    • 61
    • 14
    Kaninchen
    Klonalität
    • 125
    • 54
    Monoklonal
    Konjugat
    • 81
    • 15
    • 12
    • 11
    • 8
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD22 Antikörper ist unkonjugiert
    Applikation
    • 113
    • 71
    • 46
    • 39
    • 37
    • 19
    • 13
    • 11
    • 11
    • 8
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC)
    Immunogen
    Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary)
    Klon
    MSVA-022R
    Isotyp
    IgG
    Top Product
    Discover our top product CD22 Primärantikörper
  • Applikationshinweise
    IHC 1:100-1:200
    Kommentare

    Positive Control: Tonsil or appendix: A strong, predominantly membranous CD22 staining of the germinal centre and mantle zone B-cells as well as of few interfollicular B-cells should be seen.

    Negative Control: Tonsil or appendix: A large fraction of the interfollicular lymphocytes and all epithelial cells must not show any CD22 staining.

    Protokoll
    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Lagerung
    4 °C,-20 °C,-80 °C
    Informationen zur Lagerung
    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • Target
    CD22 (CD22 Molecule (CD22))
    Andere Bezeichnung
    CD22 (CD22 Produkte)
    Synonyme
    A530093D23 antikoerper, Lyb-8 antikoerper, Lyb8 antikoerper, SIGLEC-2 antikoerper, SIGLEC2 antikoerper, siglec-2 antikoerper, CD22 antigen antikoerper, CD22 molecule antikoerper, Cd22 antikoerper, CD22 antikoerper
    Hintergrund
    B-lymphocyte cell adhesion molecule (BL-CAM), B-cell receptor CD22, CD22, Lectin 2, Lyb8, Sialic acid-binding Ig-like lectin 2 (Siglec-2), SIGLEC2, T-cell surface antigen Leu-14,CD22 antibody validated for immunohistochemistry on 76 different Normal Tissues
    UniProt
    P20273
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