HSPD1 Antikörper (CF®405S)

Produktnummer ABIN6164322

Produktdetails anti-HSPD1 Antikörper (CF®405S)

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reaktivität: Human, Maus, Ratte, Huhn, Affe, Hund, Hamster, Rind (Kuh), Kaninchen, Schwein, Schaf, Drosophila melanogaster, Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSPD1 Antikörper ist konjugiert mit CF®405S

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)

Verwendungszweck: Mouse Monoclonal anti-HSP60 (LK1), CF405S Conjugate

Produktmerkmale: This antibody recognizes a 60 kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp?s), more appropriately called as stress response proteins (srp?s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone LK1, unlike LK2, This antibody recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human HSP60 protein

Klon: LK1

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Does not react with bacteria, helminths, or spinach, others not known
• Western blotting 0.25-0.5 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Andere Bezeichnung: HSP60 (HSPD1 Produkte)

Synonyme: CPN60 antikoerper, GROEL antikoerper, HLD4 antikoerper, HSP-60 antikoerper, HSP60 antikoerper, HSP65 antikoerper, HuCHA60 antikoerper, SPG13 antikoerper, chaperonin antikoerper, cpn60 antikoerper, groel antikoerper, hld4 antikoerper, hsp60 antikoerper, hsp65 antikoerper, spg13 antikoerper, 60kDa antikoerper, Hsp60 antikoerper, 12 antikoerper, BP5 antikoerper, CG12101 antikoerper, Cpn60 antikoerper, Dmel\\CG12101 antikoerper, Dmhsp60 antikoerper, G62 antikoerper, HSP60A antikoerper, Hsp60A antikoerper, IEF16 antikoerper, Mmp-P1 antikoerper, SSP 7506 antikoerper, d-hsp60 antikoerper, hsp60A antikoerper, l(1)10Ac antikoerper, l(1)BP5 antikoerper, l(1)G8 antikoerper, l(1)HM21 antikoerper, l(1)L12 antikoerper, l(1)dp025 antikoerper, cb863 antikoerper, fa04a05 antikoerper, fb22d10 antikoerper, fi27b05 antikoerper, id:ibd2197 antikoerper, sb:cb144 antikoerper, wu:fa04a05 antikoerper, wu:fb22d10 antikoerper, wu:fi04a12 antikoerper, wu:fi27b05 antikoerper, MIF4 antikoerper, MNA2 antikoerper, mopA antikoerper, groL antikoerper, crpA antikoerper, Hspd1-30p antikoerper, heat shock protein family D (Hsp60) member 1 antikoerper, heat shock protein family D (Hsp60) member 1 S homeolog antikoerper, 60 kDa heat shock protein, mitochondrial antikoerper, heat shock protein 1 (chaperonin) antikoerper, Heat shock protein 60A antikoerper, heat shock 60 protein 1 antikoerper, chaperone ATPase HSP60 antikoerper, molecular chaperone GroEL antikoerper, thermosome subunit antikoerper, chaperonin GroEL antikoerper, mitochondrial chaperonin antikoerper, heat shock protein family D member 1 antikoerper, HSPD1 antikoerper, hspd1.S antikoerper, hspd1 antikoerper, LOC100414401 antikoerper, Hspd1 antikoerper, Hsp60A antikoerper, HSP60 antikoerper, groEL antikoerper, MMP_RS07785 antikoerper, groEl antikoerper, LOC100136430 antikoerper

Molekulargewicht: 60 kDa

Gen-ID: 3329, 595053

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity