HSP27 Antikörper (CF®405S)
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- Target Alle HSP27 (HSPB1) Antikörper anzeigen
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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Reaktivität
- Human, Ratte, Maus, Schaf, Affe, Huhn, Schimpanse
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser HSP27 Antikörper ist konjugiert mit CF®405S
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)
- Verwendungszweck
- Mouse Monoclonal anti-HSP27 (G3.1), CF405S Conjugate
- Produktmerkmale
- This MAb reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosophila HSPs and mammalian α-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
- Klon
- G3-1
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product HSPB1 Primärantikörper
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- Applikationshinweise
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.25-0.5 μg/mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Protect from light
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- Target
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
- Andere Bezeichnung
- HSP27 (HSPB1 Produkte)
- Synonyme
- CMT2F antikoerper, HMN2B antikoerper, HS.76067 antikoerper, HSP27 antikoerper, HSP28 antikoerper, Hsp25 antikoerper, SRP27 antikoerper, 27kDa antikoerper, Hsp27 antikoerper, cb153 antikoerper, cb660 antikoerper, hsp1 antikoerper, hsp25 antikoerper, hsp27 antikoerper, id:ibd2821 antikoerper, sb:cb660 antikoerper, zgc:103437 antikoerper, HSPB1 antikoerper, cmt2f antikoerper, hs.76067 antikoerper, hsp28 antikoerper, heat shock protein family B (small) member 1 antikoerper, heat shock protein 1 antikoerper, heat shock protein, alpha-crystallin-related, 1 antikoerper, heat shock protein family B (small) member 1 L homeolog antikoerper, HSPB1 antikoerper, Hspb1 antikoerper, hspb1 antikoerper, hspb1.L antikoerper
- Molekulargewicht
- 27 kDa
- Gen-ID
- 3315, 520973
- UniProt
- P04792
- Pathways
- MAPK Signalweg, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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