CD53 Antikörper (CF®647)
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- Target Alle CD53 Antikörper anzeigen
- CD53
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD53 Antikörper ist konjugiert mit CF®647
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Applikation
- Flow Cytometry (FACS), Immunofluorescence (IF)
- Verwendungszweck
- Mouse Monoclonal anti-CD53 (63-5A3), CF647 Conjugate
- Produktmerkmale
- This antibody recognizes a protein of 33-55 kDa, identified as CD53 (Workshop V, Code CD53.1). CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Human Sezary cells
- Klon
- 63-5A3
- Isotyp
- IgG2b kappa
- Top Product
- Discover our top product CD53 Primärantikörper
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- Applikationshinweise
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Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Human tonsil and lymph node.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Protect from light
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- Target
- CD53
- Andere Bezeichnung
- CD53 (CD53 Produkte)
- Synonyme
- AI323659 antikoerper, Ox-44 antikoerper, Tspan25 antikoerper, MOX44 antikoerper, TSPAN25 antikoerper, OX44 antikoerper, CD53 antigen antikoerper, CD53 molecule antikoerper, Cd53 molecule antikoerper, Cd53 antikoerper, CD53 antikoerper
- Hintergrund
- CD53 glycoprotein, CD53 tetraspan antigen, Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, Tetraspanin-25 (Tspan-25), TSPAN25
- Molekulargewicht
- 33-55 kDa
- Gen-ID
- 963, 443057
- UniProt
- P19397
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