HIF1A Antikörper (AA 530-825) (Biotin)

Produktnummer ABIN609021

Produktdetails anti-HIF1A Antikörper (Biotin)

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Bindungsspezifität: AA 530-825

Reaktivität: Human, Maus, Ratte, Primate

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HIF1A Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: Recognizes mouse HIF-1 alpha. Species cross-reactivity: rat, human, primate. Antibody has been tested in Western Blot, recognizing a band in hypoxic samples at ~115kD. Rat retina and brain cortex have been tested. Rat brain cortex is a bit dirty but this antibody does detect upregulation of HIF-1 alpha in hypoxic samples.

Aufreinigung: Immunoaffinity purified

Immunogen: A fusion protein including aa530-825 of the mouse HIF-1 alpha protein.

Type of Immunogen: Fusion protein

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 - 10 μg/mL), WB (1:500 - 1:2000)

Usage: Suitable for Western Blot and Immunohistochemistry. Not recommended for Immunoprecipitation. Western Blot: 1:500-1:2000. Immunohistochemistry (regular/paraffin): 5-10 μg/mL. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, no preservatives added.

Konservierungsmittel: Without preservative

Handhabung: avoid freeze thaw cycles. Store undiluted.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Details zu HIF1A

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Andere Bezeichnung: HIF1A / HIF1 Alpha (HIF1A Produkte)

Synonyme: hif1a antikoerper, AA959795 antikoerper, HIF1alpha antikoerper, MOP1 antikoerper, bHLHe78 antikoerper, HIF-1A antikoerper, HIF-1alpha antikoerper, HIF1 antikoerper, HIF1-ALPHA antikoerper, PASD8 antikoerper, HIF1-alpha antikoerper, hif-1a antikoerper, hypoxia inducible factor 1 alpha antikoerper, hypoxia inducible factor 1, alpha subunit antikoerper, hypoxia inducible factor 1 alpha subunit antikoerper, HIF (Hypoxia Inducible Factor) homologa antikoerper, hif-1a antikoerper, Hif1a antikoerper, HIF1A antikoerper, hif1a antikoerper, hif-1 antikoerper

Hintergrund: Name/Gene ID: HIF1A

Synonyms: HIF1A, ARNT-interacting protein, ARNT interacting protein, BHLHe78, HIF1 Alpha, Hypoxia-inducible factor1alpha, HIF-1A, HIF1, MOP1, Member of PAS superfamily 1, PASD8, HIF-1-alpha, HIF-1alpha, HIF1-ALPHA, Member of PAS protein 1

Gen-ID: 3091

UniProt: Q16665

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effekt