GNAT3 Antikörper (AA 304-318)

Produktnummer ABIN605025

Produktdetails anti-GNAT3 Antikörper

Target: GNAT3 (Guanine Nucleotide Binding Protein, alpha Transducing 3 (GNAT3))

Bindungsspezifität: AA 304-318

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Hamster, Pferd, Affe, Schwein, Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GNAT3 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human GNAT3.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum (100%) Bat, Lizard (93%) Platypus (87%) Turkey, Chicken (80%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-KNQFLDLNLKKEDKE from an internal region of human GNAT3 (NP_001095856.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum (100%), Bat, Lizard (93%), Platypus (87%), Turkey, Chicken (80%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 27kD band in Human Colon and Testis lysates and in cell lines NIH 3T3, A431 and Kelly after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.4kD according to NP_001095856.1. The 27kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu GNAT3

Target: GNAT3 (Guanine Nucleotide Binding Protein, alpha Transducing 3 (GNAT3))

Andere Bezeichnung: GNAT3 / Gustducin (GNAT3 Produkte)

Synonyme: GDCA antikoerper, Ggust antikoerper, Gtn antikoerper, gnat-3 antikoerper, G protein subunit alpha transducin 3 antikoerper, guanine nucleotide binding protein, alpha transducing 3 antikoerper, GNAT3 antikoerper, Gnat3 antikoerper

Hintergrund: Name/Gene ID: GNAT3

Synonyms: GNAT3, Gustducin, Gustducin alpha-3 chain, GDCA

Gen-ID: 346562

NCBI Accession: NP_001095856

UniProt: A8MTJ3

Pathways: Peptide Hormone Metabolism, G-protein mediated Events, Phototransduction