IL1RN Antikörper

Produktnummer ABIN5706884

Produktdetails anti-IL1RN Antikörper

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL1RN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktmerkmale:

Synonyms: IL1RA antibody, IL1RN antibody, IL1RT1 antibody, Interleukin 1 receptor alpha type 1 antibody, Interleukin 1 receptor antagonist protein (IRAP) antibody, rabbit anti-IL-1 Receptor Antagonist Antibody, rabbit anti-1 receptor antagonist protein antibody, rabbit anti-IRAP antibody

Background: Interleukin 1 receptor antagonist is secreted by various types of cells including immune, epithelial and adipocytes. IL 1Ra is used to treat rheumatoid arthritis. Anti-IL-1 RA antibody is ideal for investigators involved in Cancer and Immunology research.

Gene Name: IL1RA

Aufreinigung: This is an IgG preparation of whole rabbit serum purified by DEAE chromatography.  In general, this antibody also detects primate IL-1Ra in the same formats using similar dilutions, but not mouse or rabbit IL-1Ra.  The antibody does not recognize human IL-1a, IL-1b or IL-1 receptor type I and type II.  This antibody is primarily directed against the secreted form of IL-1Ra (also termed sIL-1Ra) but recognizes the native (non-denatured) as well as denatured intracellular form of IL-1Ra (also termed icIL-1Ra).  IcIL-1Ra is found in many cells as constitutively expressed protein, particularly epithelial cells and keratinocytes.  This antibody does not distinguish between sIL-1Ra and icIL-1Ra in ELISA formats or by immunohistochemistry.  When used in immunoblot analysis, it will recognize 17,000 MW IL-1Ra in supernatants released from supernatants or lysates of  2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC).  PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS.  However, in lysates of PBMC, icIL-1Ra is also found after 48-72 hours of culture.  In monocytic cells, the initial IL-1Ra transcript is for sIL-1Ra which can be found almost exclusively intracellularly.

Immunogen:

Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human sIL-1Ra produced in E.coli.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This IgG fraction antibody of anti-Human IL-1 Receptor Antagonist has been tested for use in neutralizations, ELISA,  radioimmunoassays, western blotting, immunohistochemistry and flow cytometry.  Reactivity in other immunoassays is unknown.This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (code # 611-1302) and TMB as a substrate.  A working dilution range of 1:400 to 1:800 is suggested for this application. This product has been assayed by ELISA against IL-1Receptor Antagonist using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature.  A working dilution range of 1:500 to 1:1,000 is suggested for this product.  For use in ELISA formats, this antibody is  best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This product has been assayed by immunohistochemistry.  A dilution of 1:400 or greater is suggested for this immunoassay.  Either paraffin fixation or cryofixation can be used for immunohistochemistry.  This product has been assayed by radioimmunoassay against antigen.  A dilution of 1:8,000 is suggested for this immunoassay.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:1,000 - 1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 12 months

Details zu IL1RN

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Andere Bezeichnung: IL-1 Receptor Antagonist (IL1RN Produkte)

Synonyme: DIRA antikoerper, ICIL-1RA antikoerper, IL-1RN antikoerper, IL-1ra antikoerper, IL-1ra3 antikoerper, IL1F3 antikoerper, IL1RA antikoerper, IRAP antikoerper, MVCD4 antikoerper, IRAP1 antikoerper, IL1RN antikoerper, IL-1RA antikoerper, F630041P17Rik antikoerper, interleukin 1 receptor antagonist antikoerper, interleukin 1 receptor antagonist-like antikoerper, IL1RN antikoerper, Il1rn antikoerper, IL1RNL antikoerper

Gen-ID: 3557

UniProt: P18510

Pathways: NF-kappaB Signalweg, Hormone Transport, Cancer Immune Checkpoints