IGFBP5 Antikörper (Biotin)

Produktnummer ABIN5690526

Produktdetails anti-IGFBP5 Antikörper (Biotin)

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IGFBP5 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-BP5. Human IGF-BP5 specific antibody was purified by affinity chromatography and then biotinylated.

Anwendungsinformationen

Applikationshinweise: ELISA:
Direct:
To detect hIGF-BP5 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP5.

Sandwich
To detect hIGF-BP5 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IGF-BP5 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP5.

Western Blot:
To detect hIGF-BP5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: IGF-BP5 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu IGFBP5

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Andere Bezeichnung: IGF-BP5 (IGFBP5 Produkte)

Synonyme: IBP5 antikoerper, AI256729 antikoerper, AW208790 antikoerper, IGFBP-5 antikoerper, IGFBP-5P antikoerper, IGF-BP5 antikoerper, igfbp5 antikoerper, ibp5 antikoerper, IBP-5 antikoerper, xIGFBP-5 antikoerper, IGFBP5 antikoerper, igfbp5b antikoerper, zgc:165472 antikoerper, insulin like growth factor binding protein 5 antikoerper, insulin-like growth factor binding protein 5 antikoerper, insulin like growth factor binding protein 5 L homeolog antikoerper, insulin-like growth factor binding protein 5b antikoerper, insulin-like growth factor-binding protein 5 antikoerper, insulin-like growth factor binding protein 5a antikoerper, IGFBP5 antikoerper, Igfbp5 antikoerper, igfbp5.L antikoerper, igfbp5b antikoerper, igfbp5 antikoerper, LOC100194500 antikoerper, igfbp5a antikoerper

Hintergrund: IGF-BP5 is one of a family of proteins that bind to insulin-like growth factors (IGFs). IGFB-P5 is a multifunctional protein which acts not only as a traditional binding protein but also functions as a growth factor independent of IGFs to stimulate bone formation. Currently there are seven named IGF-BPs that form high affinity complexes with both IGFI and IGFII. IGF-BP5 is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGFI on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGF-BP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGF-BP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development.

Gen-ID: 3488

UniProt: P24593

Pathways: WNT Signalweg, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Growth Factor Binding