TNFSF14 Antikörper (Biotin)

Produktnummer ABIN5689060

Produktdetails anti-TNFSF14 Antikörper (Biotin)

Target: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNFSF14 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Aufreinigung: Anti-hLIGHT specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hLIGHT (human LIGHT).

Anwendungsinformationen

Applikationshinweise: ELISA:

To detect hLIGHT by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15-0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hLIGHT.

Sandwich:

To detect hLIGHT by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human LIGHT (XP-5215) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLIGHT.

Western Blot:

To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: LIGHT antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu TNFSF14

Target: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Andere Bezeichnung: LIGHT (TNFSF14 Produkte)

Synonyme: TNFSF14 antikoerper, CD258 antikoerper, HVEML antikoerper, LIGHT antikoerper, LTg antikoerper, TR2 antikoerper, HVEM-L antikoerper, Ly113 antikoerper, TNF superfamily member 14 antikoerper, tumor necrosis factor (ligand) superfamily, member 14 antikoerper, TNFSF14 antikoerper, Tnfsf14 antikoerper

Hintergrund: LIGHT is a type II membrane protein belonging to the TNF family of membrane-anchored ligands. It is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells.

Gen-ID: 8740

UniProt: O43557

Pathways: Cancer Immune Checkpoints