IL-3 Antikörper (Biotin)

Produktnummer ABIN5689042

Produktdetails anti-IL-3 Antikörper (Biotin)

Target: IL-3 (Interleukin 3 (IL-3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-3 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Aufreinigung: Anti-hIL-3 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-3 (human IL-3).

Anwendungsinformationen

Applikationshinweise: ELISA:

To detect hIL-3 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-3.

Sandwich:

To detect hIL-3 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-3 (XP-5185) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-3.

Western Blot:

To detect hIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: IL-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Andere Bezeichnung: IL-3 (IL-3 Produkte)

Synonyme: BPA antikoerper, Csfmu antikoerper, HCGF antikoerper, Il-3 antikoerper, MCGF antikoerper, PSF antikoerper, IL-3 antikoerper, MULTI-CSF antikoerper, interleukin 3 antikoerper, interleukin 3 (colony-stimulating factor, multiple) antikoerper, Il3 antikoerper, IL3 antikoerper

Hintergrund: The pleiotropic IL-3 (Interleukin 3) is a 15  kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL-3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL-3 has also been shown to protect mast cells from undergoing apoptosis.

Gen-ID: 3562

UniProt: P08700

Pathways: JAK-STAT Signalweg, Regulation of Carbohydrate Metabolic Process, Autophagie