MAPKAP Kinase 5 Antikörper (Internal Region)

Produktnummer ABIN5687996

Produktdetails anti-MAPKAP Kinase 5 Antikörper

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MAPKAP Kinase 5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Aufreinigung: MK5 / MAPKAPK5 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: MK5 / MAPKAPK5 Antibody was raised against synthetic peptide PQVLEAQRRHQKEKS found at an internal region of MK5 / MAPKAPK5.

Anwendungsinformationen

Applikationshinweise: ELISA: antibody detection limit dilution 1:64000. Western Blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP , only the expected band was observed in the MK5 lane after EB11928 labeling. Data obtained from Dr. M. B. Menon, Inst. Bioche Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear staining in the DCT. Recommended concentration, 4-8μ,g/mL. Immunoprecipitation:MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 μg EB11927 or EB11928. Western blots of

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 500 μg/mL

Buffer: MK5 / MAPKAPK5 is supplied in Tris saline, 0.02 % sodium azide,  pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Details zu MAPKAP Kinase 5

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Andere Bezeichnung: MK5 / MAPKAPK5 (MAPKAPK5 Produkte)

Synonyme: MAPKAPK5 antikoerper, MK5 antikoerper, PRAK antikoerper, MAPKAP-K5 antikoerper, MK-5 antikoerper, mitogen-activated protein kinase-activated protein kinase 5 antikoerper, MAP kinase-activated protein kinase 5 antikoerper, MAPKAPK5 antikoerper, mapkapk5 antikoerper, Mapkapk5 antikoerper

Gen-ID: 8550

NCBI Accession: NP_003659, NP_620777

UniProt: Q8IW41

Pathways: MAPK Signalweg