COL2 Antikörper

Produktnummer ABIN5680225

Produktdetails anti-COL2 Antikörper

Target: COL2 (Collagen, Type II (COL2))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COL2 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)

Spezifität: Bovine collagen type II 100 % , bovine collagen XI <3.0 %, bovine collagen I and IX <0.1 % (RIA at 1:200 dilution) (cross-reacting with horse and pig)

Kreuzreaktivität: Pferd, Schwein

Kreuzreaktivität (Details): Cattle, (cross-reacting with horse and pig)

Produktmerkmale: Type II collagen is an alpha1(II)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in cartilage and various tumours. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Bovine collagen type II 100 % , bovine collagen XI <3.0 %, bovine collagen I and IX <0.1 % (RIA at 1:200 dilution).

Aufreinigung: Affinity purified

Immunogen: Purified collagen type II from bovine cartilage

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Bovine cartilage,IHC(P), IFA, ELISA, RIA,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA 1:40, IHC(P) 1:100 - 1:500, ELISA ? 1:200 (OD ? 500)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 1 mg/mL

Buffer: phosphate buffered solution, no BSA and preservative added!

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Details zu COL2

Target: COL2 (Collagen, Type II (COL2))

Andere Bezeichnung: Collagen Type II (COL2 Produkte)

Synonyme: PT antikoerper, RPRGL2 antikoerper, THPH1 antikoerper, Cf-2 antikoerper, Cf2 antikoerper, FII antikoerper, thrombin antikoerper, prothrombin antikoerper, CAP18 antikoerper, Cnlp antikoerper, Cramp antikoerper, FALL39 antikoerper, MCLP antikoerper, coagulation factor II, thrombin antikoerper, coagulation factor II antikoerper, cathelicidin antimicrobial peptide antikoerper, Cuticle collagen 2 antikoerper, collagen type II alpha 1 chain antikoerper, F2 antikoerper, Camp antikoerper, col-2 antikoerper, COL2A1 antikoerper