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COL2 Antikörper

COL2 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Säugetier, Schaf WB, ELISA, IHC, IP Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN5596824
  • Target Alle COL2 Antikörper anzeigen
    COL2 (Collagen, Type II (COL2))
    Reaktivität
    • 44
    • 32
    • 28
    • 23
    • 18
    • 12
    • 11
    • 3
    Human, Maus, Ratte, Rind (Kuh), Säugetier, Schaf
    Wirt
    • 41
    • 13
    • 10
    • 4
    Kaninchen
    Klonalität
    • 55
    • 13
    Polyklonal
    Konjugat
    • 34
    • 12
    • 7
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser COL2 Antikörper ist unkonjugiert
    Applikation
    • 50
    • 37
    • 21
    • 21
    • 16
    • 14
    • 13
    • 13
    • 9
    • 6
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Kreuzreaktivität (Details)
    Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
    Aufreinigung
    Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
    Immunogen

    Immunogen: Collagen Type II from human knee cartilage and bovine nasal cartilage.

    Immunogen Type: Native Protein

    Isotyp
    IgG
    Top Product
    Discover our top product COL2 Primärantikörper
    Produktspezifische Information

    Für welche Zwecke kann der COL2-Antikörper ABIN5596824 verwendet werden? Dieser polyklonale, unkonjugierte Kaninchen-COL2-Antikörper eignet sich für den Nachweis von Kollagen Typ 2 (COL2) mittels ELISA, Immunhistochemie, Immunpräzipitation und Western Blotting. Der Antikörper erkennt COL2 in Mensch, Maus, Ratte, Rind, Schaf und anderen Säugetieren.

    Welche Validierungsdaten liegen für diesen COL2-Antikörper vor? Das Produkt wurde von NanoTemper Technologies durch Bestimmung des Entfaltungsprofils unabhängig validiert. Es sind derzeit 2 Produktbilder verfügbar, die die Leistungsfähigkeit des COL2-Antikörpers zeigen. Nutzen Sie unseren Antikörper, um COL2 zuverlässig nachzuweisen.

    Was ist die Funktion von COL2? Kollagen Typ 2 (COL2) ist spezifisch für Knorpelgewebe. Es ist wichtig für die normale embryonale Entwicklung des Skeletts, für das lineare Wachstum und für die Fähigkeit des Knorpels, Druckkräften zu widerstehen.

  • Applikationshinweise

    Immunohistochemistry Dilution: 1:50 - 1:400

    Application Note: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.

    Western Blot Dilution: 1:1,000 - 1:10,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:50,000

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #104083 (Unfolding Profile)
    'Independent Validation' Siegel
    by
    NanoTemper Technologies
    No.
    #104083
    Datum
    23.07.2019
    Antigen
    COL2
    Chargennummer
    41639
    Validierte Anwendung
    Unfolding Profile
    Positivkontrolle
    ABIN5596824
    Negativkontrolle
    Bewertung

    Passed. ABIN5596824 showed Ti at 78.9°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    Sekundärantikörper
    Full Protocol
    • Dilute ABIN5596824 in PBS buffer (Roth, 1058.1, Lot 285231988) to get a final volume of 30µl at a concentration of 0.1µM.
    • Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
    • Run Tycho measurement.
    Anmerkungen

    Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.

  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

    Stabilizer: None

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
    Haltbarkeit
    12 months
  • Target
    COL2 (Collagen, Type II (COL2))
    Andere Bezeichnung
    Collagen Type II (COL2 Produkte)
    Synonyme
    PT antikoerper, RPRGL2 antikoerper, THPH1 antikoerper, Cf-2 antikoerper, Cf2 antikoerper, FII antikoerper, thrombin antikoerper, prothrombin antikoerper, CAP18 antikoerper, Cnlp antikoerper, Cramp antikoerper, FALL39 antikoerper, MCLP antikoerper, coagulation factor II, thrombin antikoerper, coagulation factor II antikoerper, cathelicidin antimicrobial peptide antikoerper, Cuticle collagen 2 antikoerper, collagen type II alpha 1 chain antikoerper, F2 antikoerper, Camp antikoerper, col-2 antikoerper, COL2A1 antikoerper
    Hintergrund

    Synonyms: Cartilage collagen antibody, Chondrocalcin antibody, COL11A3 antibody, COL2A1 antibody

    Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. We extensively purify collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.

    Gene Name: COL2A1

    Gen-ID
    1280
    UniProt
    P02458
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