SLC2A2 Antikörper (C-Term)

Produktnummer ABIN5596790

Produktdetails anti-SLC2A2 Antikörper

Target: SLC2A2 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 2 (SLC2A2))

Bindungsspezifität: C-Term

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC2A2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), FLISA

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Glut2 from mouse and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Aufreinigung: This affinity-purified Glut-2 antibody is directed against Glut2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen:

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of mouse Glut2 protein.

Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 4 μg/mL

Application Note: Anti-Glut-2 antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57.1 kDa in size corresponding to Glut2 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

Western Blot Dilution: 1:500-1:1000

FLISA Dilution: 1

ELISA Dilution: 1:30,000-1:90:000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-SLC2A2 Antikörper (ABIN5596790)

Liang, Yee, Chien, Chen, Luo, Zou, Piao, Mifune, Chen, Calvert, King, Norheim, Abad, Krauss, Giacomini: "Organic cation transporter 1 (OCT1) modulates multiple cardiometabolic traits through effects on hepatic thiamine content." in: PLoS biology, Vol. 16, Issue 4, pp. e2002907, (2019) (PubMed).

Chhabra, Morgan, Tooke, Adams, Rahmouni, Low: "Reduced renal sympathetic nerve activity contributes to elevated glycosuria and improved glucose tolerance in hypothalamus-specific Pomc knockout mice." in: Molecular metabolism, Vol. 6, Issue 10, pp. 1274-1285, (2018) (PubMed).

Chhabra, Adams, Fagel, Lam, Qi, Rubinstein, Low: "Hypothalamic POMC Deficiency Improves Glucose Tolerance Despite Insulin Resistance by Increasing Glycosuria." in: Diabetes, Vol. 65, Issue 3, pp. 660-72, (2016) (PubMed).

Details zu SLC2A2

Target: SLC2A2 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 2 (SLC2A2))

Andere Bezeichnung: Glut2 (SLC2A2 Produkte)

Synonyme: glut2 antikoerper, wu:fb62h10 antikoerper, slc2a2 antikoerper, MGC83262 antikoerper, SLC2A2 antikoerper, GLUT2 antikoerper, GTT2 antikoerper, Glut2 antikoerper, AI266973 antikoerper, Glut-2 antikoerper, solute carrier family 2 (facilitated glucose transporter), member 2 antikoerper, solute carrier family 2 member 2 L homeolog antikoerper, solute carrier family 2 member 2 antikoerper, solute carrier family 2, facilitated glucose transporter member 2 antikoerper, slc2a2 antikoerper, slc2a2.L antikoerper, SLC2A2 antikoerper, LOC100534484 antikoerper, Slc2a2 antikoerper

Hintergrund:

Synonyms: Solute carrier family 2, facilitated glucose transporter member 2, Glucose transporter type 2, liver-GLUT-2, Slc2a2, Glut2 Antibody

Background: The Anti-Glut2 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). The glucose transporter GLUT2 is a transmembrane carrier protein that allows protein facilitated glucose movement across cell membranes. GLUT2 is expressed in the plasma membranes of the liver, intestine, renal tubular cells, pancreatic islet beta cells, as well as in the portal and hypothalamic areas. Due to its low affinity and high capacity, GLUT2 transports dietary sugars, glucose, galactose and fructose in high concentrations, displaying large bidirectional fluxes in and out of cells. In pancreatic beta cells, GLUT2 is essential for glucose-stimulated insulin secretion. GLUT2 expression is necessary for the physiological control of glucose-sensitive genes, and its inactivation in the liver leads to impaired glucose-stimulated insulin secretion. In the nervous system, GLUT2-dependent glucose sensing regulates feeding, thermoregulation and pancreatic islet cell mass and function, as well as sympathetic and parasympathetic activities. In humans, inactivating mutations in GLUT2 cause Fanconi-Bickel syndrome, which is characterized by hepatomegaly and kidney disease. Anti-Glut2 is ideal for researchers interested in studying glucose transport mediated by Glut2 protein in the fields of diabetes, obesity, metabolism, and neuroscience research.

Gene Name: Slc2a2

Gen-ID: 20526

UniProt: P14246

Pathways: Warburg Effekt