Nemo-Like Kinase Antikörper (N-Term)

Produktnummer ABIN5516121

Produktdetails anti-Nemo-Like Kinase Antikörper

Target: Nemo-Like Kinase (NLK)

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nemo-Like Kinase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: SSAAAAAAAA AAAAAMLNPG QQQPYFPSPA PGQAPGPAAA APAQVQAAAA

Produktmerkmale: This is a rabbit polyclonal antibody against NLK. It was validated on Western Blot.

Aufreinigung: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human NLK

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu Nemo-Like Kinase

Target: Nemo-Like Kinase (NLK)

Andere Bezeichnung: NLK (NLK Produkte)

Synonyme: Amf antikoerper, Gpi1 antikoerper, AI194375 antikoerper, nlk antikoerper, nlkb antikoerper, zgc:111875 antikoerper, Xnlk antikoerper, nlk1 antikoerper, nlk2 antikoerper, NLK antikoerper, nemo-like antikoerper, RGD1561602 antikoerper, glucose-6-phosphate isomerase antikoerper, nemo like kinase antikoerper, nemo-like kinase, type 1 antikoerper, nemo-like kinase L homeolog antikoerper, Nemo-Like kinase antikoerper, nemo-like kinase antikoerper, Gpi antikoerper, NLK antikoerper, Nlk antikoerper, nlk1 antikoerper, nlk.L antikoerper, nlk antikoerper, nemo-like antikoerper

Hintergrund: NLK is a serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. It is a positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK- SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. It binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. It is a negative regulator of the Notch signaling pathway. It binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1.

Alias Symbols: NLK, LAK1 {ECO:0000312|EMBL:AAD56013.1

Protein Interaction Partner: KRTAP5-6, KRTAP10-3, KRTAP4-2, QRICH1, ZHX3, KRTAP5-9, GRN, TP53, MDM2, STAT5A, LEF1, CNOT2, SMAD4, KPNB1, BACH1, TNKS1BP1, FAM222A, C2orf44, CEP97, RNF219, FAM222B, CNOT11, NLK, PASK, SHANK2, UBAP2L, CCP110, TRPS1, TLE3, SKI, RANGAP1, RANBP2, PKM, CNOT3,

Protein Size: 527

Gen-ID: 51701

NCBI Accession: NP_057315

UniProt: Q9UBE8

Pathways: Ubiquitin Proteasome Pathway