POLK
Reaktivität: Human
WB, ELISA
Wirt: Ziege
Polyclonal
unconjugated
Applikationshinweise
ELISA (1:2000-1:10000) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Uljon, Johnson, Edwards, Prakash, Prakash, Aggarwal: "Crystal structure of the catalytic core of human DNA polymerase kappa." in: Structure (London, England : 1993), Vol. 12, Issue 8, pp. 1395-404, (2004) (PubMed).
Yasui, Suzuki, Miller, Matsuda, Matsui, Shibutani: "Translesion synthesis past 2'-deoxyxanthosine, a nitric oxide-derived DNA adduct, by mammalian DNA polymerases." in: Journal of molecular biology, Vol. 344, Issue 3, pp. 665-74, (2004) (PubMed).
Haracska, Prakash, Prakash: "A mechanism for the exclusion of low-fidelity human Y-family DNA polymerases from base excision repair." in: Genes & development, Vol. 17, Issue 22, pp. 2777-85, (2003) (PubMed).