Cyclin E2 Antikörper

Produktnummer ABIN498694

Produktdetails anti-Cyclin E2 Antikörper

Target: Cyclin E2 (CCNE2)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin E2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of Cyclin E2 protein. (region surrounding asn387)

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Cyclin E2

Target: Cyclin E2 (CCNE2)

Andere Bezeichnung: Cyclin E2 (CCNE2 Produkte)

Synonyme: ccne2 antikoerper, MGC53684 antikoerper, MGC132360 antikoerper, cb165 antikoerper, sb:cb165 antikoerper, zgc:86724 antikoerper, CCNE2 antikoerper, cyce2 antikoerper, CYCE2 antikoerper, cyclin E2 antikoerper, cyclin E2 L homeolog antikoerper, cyclin E1 L homeolog antikoerper, CCNE2 antikoerper, ccne2.L antikoerper, ccne2 antikoerper, ccne1.L antikoerper, Ccne2 antikoerper

Hintergrund: Cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. Cyclin E2 is a cyclin E-related protein that specifically interacts with Cdk2 and Cdk3 and with p27 and p21. Cyclin E2 expression peaks at the G1/S phase transition of the cell cycle, in parallel with cyclin E. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells.Synonyms: CCNE2, Cyclin-E2, G1/S-specific cyclin-E2

Molekulargewicht: approx. 46 kDa

Gen-ID: 9134

NCBI Accession: NP_477097

UniProt: O96020

Pathways: Zellzyklus, Mitotic G1-G1/S Phases